TY - JOUR
T1 - Single-molecule mechanochemical sensing using DNA origami nanostructures
AU - Koirala, Deepak
AU - Shrestha, Prakash
AU - Emura, Tomoko
AU - Hidaka, Kumi
AU - Mandal, Shankar
AU - Endo, Masayuki
AU - Sugiyama, Hiroshi
AU - Mao, Hanbin
PY - 2014/7/28
Y1 - 2014/7/28
N2 - While single-molecule sensing offers the ultimate detection limit, its throughput is often restricted as sensing events are carried out one at a time in most cases. 2D and 3D DNA origami nanostructures are used as expanded single-molecule platforms in a new mechanochemical sensing strategy. As a proof of concept, six sensing probes are incorporated in a 7-tile DNA origami nanoassembly, wherein binding of a target molecule to any of these probes leads to mechanochemical rearrangement of the origami nanostructure, which is monitored in real time by optical tweezers. Using these platforms, 10 pM platelet-derived growth factor (PDGF) are detected within 10 minutes, while demonstrating multiplex sensing of the PDGF and a target DNA in the same solution. By tapping into the rapid development of versatile DNA origami nanostructures, this mechanochemical platform is anticipated to offer a long sought solution for single-molecule sensing with improved throughput.
AB - While single-molecule sensing offers the ultimate detection limit, its throughput is often restricted as sensing events are carried out one at a time in most cases. 2D and 3D DNA origami nanostructures are used as expanded single-molecule platforms in a new mechanochemical sensing strategy. As a proof of concept, six sensing probes are incorporated in a 7-tile DNA origami nanoassembly, wherein binding of a target molecule to any of these probes leads to mechanochemical rearrangement of the origami nanostructure, which is monitored in real time by optical tweezers. Using these platforms, 10 pM platelet-derived growth factor (PDGF) are detected within 10 minutes, while demonstrating multiplex sensing of the PDGF and a target DNA in the same solution. By tapping into the rapid development of versatile DNA origami nanostructures, this mechanochemical platform is anticipated to offer a long sought solution for single-molecule sensing with improved throughput.
KW - DNA nanotechnology
KW - DNA origami nanostructures
KW - mechanochemical sensing
KW - optical tweezers
KW - single-molecule techniques
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U2 - 10.1002/anie.201404043
DO - 10.1002/anie.201404043
M3 - Article
AN - SCOPUS:84905389596
SN - 1433-7851
VL - 53
SP - 8137
EP - 8141
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 31
ER -