Singlet oxygen generation in the superoxide reaction

Y. Mao, L. Zang, X. Shi

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


ESR spin trapping was utilized to study the singlet oxygen (1O2) generation in the reaction of superoxide (O2-) with H2O2. The spin trap used was 2,2,6,6-tetramethyl-4-piperdone. Incubation of xanthine, xanthine oxidase and H2O2 generated 1O2 spin adduct signal. Omission of xanthine, xanthine oxidase or H2O2 caused a sharp decrease in 1O2 generation. 1O2 scavenger, sodium azide, inhibited 1O2 generation while .OH scavenger, ethanol, only slightly decreased the signal intensity. Potassium superoxide (KO2) decomposition generated 1O2. Catalase and sodium azide inhibited 1O2- generation and H2O2 enhanced it. The results demonstrate that O2 is capable of generating 1O2 upon reaction with H2O2.

Original languageEnglish
Pages (from-to)227-232
Number of pages6
JournalBiochemistry and Molecular Biology International
Issue number1
StatePublished - 1995

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics


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