Site-specific effects of dietary salt intake on guanylin and uroguanylin mRNA expression in rat intestine

S. L. Carrithers; B. A. Jackson; W. Y. Cai; R. N. Greenberg; C. E. Ott

doi:10.1016/S0167-0115(02)00069-1

Site-specific effects of dietary salt intake on guanylin and uroguanylin mRNA expression in rat intestine

S. L. Carrithers, B. A. Jackson, W. Y. Cai, R. N. Greenberg, C. E. Ott

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Guanylin and uroguanylin are newly discovered intestinal peptides that have been shown to affect NaCl transport in both the intestine and kidney. The present study tests the hypothesis that guanylin and uroguanylin mRNA expression in each major region of the intestine is regulated by NaCl intake. Semiquantitative multiplex RT-PCR analysis was used to determine the molecular expression of guanylin and uroguanylin in the duodenum, jejunum, ileum, and colon in rats maintained on low (LS), normal (NS), or high (HS) NaCl intake for 4 days. LS intake reduced the expression of uroguanylin, and to a lesser degree, guanylin mRNA in all intestinal segments compared to NS intake. The duodenum was the site of the greatest decrease for both. In contrast, HS intake significantly increased the expression of guanylin mRNA only in the duodenum and jejunum and had minimal effect on uroguanylin mRNA. The minimum time required for altered gene expression was determined by delivering an oral NaCl challenge directly to the gastrointestinal tract by oro-gastric administration to LS or NS animals. In LS rats, NaCl oro-gastric administration significantly increased mRNA expression of both peptides in all intestinal segments. Furthermore, the increases in guanylin and uroguanylin mRNA were detected within 4 h and plateaued by 8 h. Conversely, acute oro-gastric administration of the same NaCl solution to NS rats caused elevations of guanylin mRNA only in the duodenum and jejunum, and of uroguanylin mRNA only in the ileum and colon. In conclusion, the data demonstrate that variations in NaCl intake lead to intestinal segment-specific changes in guanylin and uroguanylin mRNA expression.

Original language	English
Pages (from-to)	87-95
Number of pages	9
Journal	Regulatory Peptides
Volume	107
Issue number	1-3
DOIs	https://doi.org/10.1016/S0167-0115(02)00069-1
State	Published - Jul 15 2002

Bibliographical note

Funding Information:
The authors would like to thank the intellectual contribution and technical assistance of Andre F. Carvalho, MD, Federal University of Ceará, Fortaleza, Brazil and graphics by Rajesh Shah, University of Kentucky, Division of Infectious Diseases. This work was supported by the Office of Research and Development, Medical Research Service, Department of Veterans Affairs, Lexington, KY (RNG, BAJ, SLC), the American Cancer Society (SLC, #85-001-13-IRG), and the National (BAJ) and OH Affiliate of the American Heart Association (CEO).

Funding

The authors would like to thank the intellectual contribution and technical assistance of Andre F. Carvalho, MD, Federal University of Ceará, Fortaleza, Brazil and graphics by Rajesh Shah, University of Kentucky, Division of Infectious Diseases. This work was supported by the Office of Research and Development, Medical Research Service, Department of Veterans Affairs, Lexington, KY (RNG, BAJ, SLC), the American Cancer Society (SLC, #85-001-13-IRG), and the National (BAJ) and OH Affiliate of the American Heart Association (CEO).

Funders	Funder number
Global CEO Initiative on Alzheimer's disease
VA Medical Research Service
American Cancer Society-Michigan Cancer Research Fund
Science of Learning Centers	85-001-13-IRG
Science of Learning Centers
U.S. Department of Veterans Affairs
American the American Heart Association
Biomedical Laboratory Research and Development, VA Office of Research and Development

Keywords

Guanylyl cyclase-C
Intestine
Kidney
Multiplex PCR
Salt intake

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

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10.1016/S0167-0115(02)00069-1

Cite this

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title = "Site-specific effects of dietary salt intake on guanylin and uroguanylin mRNA expression in rat intestine",

abstract = "Guanylin and uroguanylin are newly discovered intestinal peptides that have been shown to affect NaCl transport in both the intestine and kidney. The present study tests the hypothesis that guanylin and uroguanylin mRNA expression in each major region of the intestine is regulated by NaCl intake. Semiquantitative multiplex RT-PCR analysis was used to determine the molecular expression of guanylin and uroguanylin in the duodenum, jejunum, ileum, and colon in rats maintained on low (LS), normal (NS), or high (HS) NaCl intake for 4 days. LS intake reduced the expression of uroguanylin, and to a lesser degree, guanylin mRNA in all intestinal segments compared to NS intake. The duodenum was the site of the greatest decrease for both. In contrast, HS intake significantly increased the expression of guanylin mRNA only in the duodenum and jejunum and had minimal effect on uroguanylin mRNA. The minimum time required for altered gene expression was determined by delivering an oral NaCl challenge directly to the gastrointestinal tract by oro-gastric administration to LS or NS animals. In LS rats, NaCl oro-gastric administration significantly increased mRNA expression of both peptides in all intestinal segments. Furthermore, the increases in guanylin and uroguanylin mRNA were detected within 4 h and plateaued by 8 h. Conversely, acute oro-gastric administration of the same NaCl solution to NS rats caused elevations of guanylin mRNA only in the duodenum and jejunum, and of uroguanylin mRNA only in the ileum and colon. In conclusion, the data demonstrate that variations in NaCl intake lead to intestinal segment-specific changes in guanylin and uroguanylin mRNA expression.",

keywords = "Guanylyl cyclase-C, Intestine, Kidney, Multiplex PCR, Salt intake",

author = "Carrithers, \{S. L.\} and Jackson, \{B. A.\} and Cai, \{W. Y.\} and Greenberg, \{R. N.\} and Ott, \{C. E.\}",

note = "Funding Information: The authors would like to thank the intellectual contribution and technical assistance of Andre F. Carvalho, MD, Federal University of Cear{\'a}, Fortaleza, Brazil and graphics by Rajesh Shah, University of Kentucky, Division of Infectious Diseases. This work was supported by the Office of Research and Development, Medical Research Service, Department of Veterans Affairs, Lexington, KY (RNG, BAJ, SLC), the American Cancer Society (SLC, \#85-001-13-IRG), and the National (BAJ) and OH Affiliate of the American Heart Association (CEO).",

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doi = "10.1016/S0167-0115(02)00069-1",

language = "English",

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T1 - Site-specific effects of dietary salt intake on guanylin and uroguanylin mRNA expression in rat intestine

AU - Carrithers, S. L.

AU - Jackson, B. A.

AU - Cai, W. Y.

AU - Greenberg, R. N.

AU - Ott, C. E.

N1 - Funding Information: The authors would like to thank the intellectual contribution and technical assistance of Andre F. Carvalho, MD, Federal University of Ceará, Fortaleza, Brazil and graphics by Rajesh Shah, University of Kentucky, Division of Infectious Diseases. This work was supported by the Office of Research and Development, Medical Research Service, Department of Veterans Affairs, Lexington, KY (RNG, BAJ, SLC), the American Cancer Society (SLC, #85-001-13-IRG), and the National (BAJ) and OH Affiliate of the American Heart Association (CEO).

PY - 2002/7/15

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N2 - Guanylin and uroguanylin are newly discovered intestinal peptides that have been shown to affect NaCl transport in both the intestine and kidney. The present study tests the hypothesis that guanylin and uroguanylin mRNA expression in each major region of the intestine is regulated by NaCl intake. Semiquantitative multiplex RT-PCR analysis was used to determine the molecular expression of guanylin and uroguanylin in the duodenum, jejunum, ileum, and colon in rats maintained on low (LS), normal (NS), or high (HS) NaCl intake for 4 days. LS intake reduced the expression of uroguanylin, and to a lesser degree, guanylin mRNA in all intestinal segments compared to NS intake. The duodenum was the site of the greatest decrease for both. In contrast, HS intake significantly increased the expression of guanylin mRNA only in the duodenum and jejunum and had minimal effect on uroguanylin mRNA. The minimum time required for altered gene expression was determined by delivering an oral NaCl challenge directly to the gastrointestinal tract by oro-gastric administration to LS or NS animals. In LS rats, NaCl oro-gastric administration significantly increased mRNA expression of both peptides in all intestinal segments. Furthermore, the increases in guanylin and uroguanylin mRNA were detected within 4 h and plateaued by 8 h. Conversely, acute oro-gastric administration of the same NaCl solution to NS rats caused elevations of guanylin mRNA only in the duodenum and jejunum, and of uroguanylin mRNA only in the ileum and colon. In conclusion, the data demonstrate that variations in NaCl intake lead to intestinal segment-specific changes in guanylin and uroguanylin mRNA expression.

AB - Guanylin and uroguanylin are newly discovered intestinal peptides that have been shown to affect NaCl transport in both the intestine and kidney. The present study tests the hypothesis that guanylin and uroguanylin mRNA expression in each major region of the intestine is regulated by NaCl intake. Semiquantitative multiplex RT-PCR analysis was used to determine the molecular expression of guanylin and uroguanylin in the duodenum, jejunum, ileum, and colon in rats maintained on low (LS), normal (NS), or high (HS) NaCl intake for 4 days. LS intake reduced the expression of uroguanylin, and to a lesser degree, guanylin mRNA in all intestinal segments compared to NS intake. The duodenum was the site of the greatest decrease for both. In contrast, HS intake significantly increased the expression of guanylin mRNA only in the duodenum and jejunum and had minimal effect on uroguanylin mRNA. The minimum time required for altered gene expression was determined by delivering an oral NaCl challenge directly to the gastrointestinal tract by oro-gastric administration to LS or NS animals. In LS rats, NaCl oro-gastric administration significantly increased mRNA expression of both peptides in all intestinal segments. Furthermore, the increases in guanylin and uroguanylin mRNA were detected within 4 h and plateaued by 8 h. Conversely, acute oro-gastric administration of the same NaCl solution to NS rats caused elevations of guanylin mRNA only in the duodenum and jejunum, and of uroguanylin mRNA only in the ileum and colon. In conclusion, the data demonstrate that variations in NaCl intake lead to intestinal segment-specific changes in guanylin and uroguanylin mRNA expression.

KW - Guanylyl cyclase-C

KW - Intestine

KW - Kidney

KW - Multiplex PCR

KW - Salt intake

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SN - 0167-0115

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JO - Regulatory Peptides

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IS - 1-3

ER -

Site-specific effects of dietary salt intake on guanylin and uroguanylin mRNA expression in rat intestine

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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