TY - JOUR
T1 - Site-specific incorporation of sodium tripolyphosphate into myofibrillar protein from mantis shrimp (Oratosquilla oratoria) promotes protein crosslinking and gel network formation
AU - Chen, Jinyu
AU - Ren, Yunxia
AU - Zhang, Kunsheng
AU - Xiong, Youling L.
AU - Wang, Qing
AU - Shang, Kun
AU - Zhang, Dian
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2020/5/15
Y1 - 2020/5/15
N2 - Formation of protein gels in processed muscle foods is one of the most important functionalities. To explore the mechanisms responsible for affecting gel properties of muscle proteins by phosphates, myofibrillar protein (MP) from mantis shrimp (Oratosquilla oratoria) was treated with sodium tripolyphosphate at three pH values (7.0, 8.0, and 9.0). FTIR and UPLC-MS/MS firstly confirmed that phosphate groups were introduced to MP through C[sbnd]O[sbnd]P bonds via serine and threonine residues. The incorporation of STP caused increased electronegativity and solubility, more stable α-helix secondary conformation, and reduced tryptophan fluorescence intensity of MP, especially at pH 8.0 and 9.0. These changes led to a finer, ordered and denser three-dimensional gel network microstructure with higher gel strength and elasticity, and water-holding capacity. This study demonstrated that the introduction of phosphate groups could increase negatively charged residues in MP, enhance the crosslinks of proteins through ionic interaction, and promote gel network formation.
AB - Formation of protein gels in processed muscle foods is one of the most important functionalities. To explore the mechanisms responsible for affecting gel properties of muscle proteins by phosphates, myofibrillar protein (MP) from mantis shrimp (Oratosquilla oratoria) was treated with sodium tripolyphosphate at three pH values (7.0, 8.0, and 9.0). FTIR and UPLC-MS/MS firstly confirmed that phosphate groups were introduced to MP through C[sbnd]O[sbnd]P bonds via serine and threonine residues. The incorporation of STP caused increased electronegativity and solubility, more stable α-helix secondary conformation, and reduced tryptophan fluorescence intensity of MP, especially at pH 8.0 and 9.0. These changes led to a finer, ordered and denser three-dimensional gel network microstructure with higher gel strength and elasticity, and water-holding capacity. This study demonstrated that the introduction of phosphate groups could increase negatively charged residues in MP, enhance the crosslinks of proteins through ionic interaction, and promote gel network formation.
KW - C[sbnd]O[sbnd]P bond
KW - Gel network formation
KW - LC-MS/MS
KW - Microstructure
KW - Myofibrillar protein
KW - Sodium tripolyphosphate
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U2 - 10.1016/j.foodchem.2019.126113
DO - 10.1016/j.foodchem.2019.126113
M3 - Article
C2 - 31911356
AN - SCOPUS:85077775772
SN - 0308-8146
VL - 312
JO - Food Chemistry
JF - Food Chemistry
M1 - 126113
ER -