The purpose of this study was to assess perfusion in a free skin flap model using contrast-enhanced ultrasound (CEU), and to determine if the extent of perfusion early after venous occlusion predicted long-term flap survival. Perfusion was assessed in an autologous abdominal skin flap and adjacent healthy skin in rats using CEU imaging before venous occlusion and following reflow. Perfusion assessment was possible in all flaps and quantitative measurements of microvascular blood volume (BV) and blood velocity were expressed as a ratio to that in the healthy skin. Proximal flap BV 18 h after venous occlusion was significantly greater in those that survived (n = 4) vs. those that became necrotic (n = 6) (BV ratio 0.8 ± 0.1 vs. 0.2 ± 0.1, p = 0.0001). A BV ratio of 0.5 predicted graft viability with a sensitivity and specificity of 100%. Microvascular blood velocity at 18 h was similar in grafts that survived and those that became necrotic. Qualitative assessment of perfusion by a 'blinded' observer correlated well with quantitative data and predicted flap outcome in all cases. We conclude that skin perfusion can be assessed with CEU. Perfusion 18 h following a secondary ischemic insult in a free flap accurately predicts subsequent tissue survival in this model.
|Number of pages||6|
|Journal||Ultrasound in Medicine and Biology|
|State||Published - 2002|
Bibliographical noteFunding Information:
This study was supported by grants HL-03810, HL-48890, HL-65704 from the National Institutes of Health, Bethesda, MD. Jonathan Christiansen is the recipient of a Postdoctoral Fellowship Award from the American Heart Association, Mid-Atlantic Affiliate, Baltimore, MD. Howard Leong-Poi is the recipient of a Fellowship Training Grant from the Canadian Institutes of Health Research and the Heart and Stroke Foundation of Canada, Ottawa, ONT, Canada.
ASJC Scopus subject areas
- Radiological and Ultrasound Technology
- Acoustics and Ultrasonics