Slot-Blot Analysis of 3-Nitrotyrosine-Modified Brain Proteins

Rukhsana Sultana; D. Allan Butterfield

doi:10.1016/S0076-6879(07)00820-8

Slot-Blot Analysis of 3-Nitrotyrosine-Modified Brain Proteins

Rukhsana Sultana, D. Allan Butterfield

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

24 Scopus citations

Abstract

3-Nitrotyrosine (3-NT) is used as a biomarker of nitrosative stress. The formation of 3-NT has been reported in a number of diseases, including Alzheimer's disease, Huntington's disease, Parkinson's disease, amyotrophic lateral sclerosis, and cancer. The nitration of proteins is a reversible process, but it can induce a conformational change and thereby functional alterations of the affected protein. 3-NT measurements in biological samples are usually carried out by methodologies such as immunohistochemistry, high-performance liquid chromatography, gas chromatography, and immunochemical detection. This chapter describes the immunochemical method for the determination of protein-bound 3-NT using slot-blot analysis.

Original language	English
Title of host publication	Nitric Oxide, Part F
Editors	Enrique Cadenas, Lester Packer
Pages	309-316
Number of pages	8
DOIs	https://doi.org/10.1016/S0076-6879(07)00820-8
State	Published - 2008

Publication series

Name	Methods in Enzymology
Volume	440
ISSN (Print)	0076-6879

Bibliographical note

Funding Information:
This work was supported in part by NIH Grants AG‐10836 and AG‐05119.

ASJC Scopus subject areas

Biochemistry
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0076-6879(07)00820-8

Cite this

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abstract = "3-Nitrotyrosine (3-NT) is used as a biomarker of nitrosative stress. The formation of 3-NT has been reported in a number of diseases, including Alzheimer's disease, Huntington's disease, Parkinson's disease, amyotrophic lateral sclerosis, and cancer. The nitration of proteins is a reversible process, but it can induce a conformational change and thereby functional alterations of the affected protein. 3-NT measurements in biological samples are usually carried out by methodologies such as immunohistochemistry, high-performance liquid chromatography, gas chromatography, and immunochemical detection. This chapter describes the immunochemical method for the determination of protein-bound 3-NT using slot-blot analysis.",

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AB - 3-Nitrotyrosine (3-NT) is used as a biomarker of nitrosative stress. The formation of 3-NT has been reported in a number of diseases, including Alzheimer's disease, Huntington's disease, Parkinson's disease, amyotrophic lateral sclerosis, and cancer. The nitration of proteins is a reversible process, but it can induce a conformational change and thereby functional alterations of the affected protein. 3-NT measurements in biological samples are usually carried out by methodologies such as immunohistochemistry, high-performance liquid chromatography, gas chromatography, and immunochemical detection. This chapter describes the immunochemical method for the determination of protein-bound 3-NT using slot-blot analysis.

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Slot-Blot Analysis of 3-Nitrotyrosine-Modified Brain Proteins

Abstract

Publication series

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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