Small RNA (sRNA) expression in the chorioallantois, endometrium and serum of mares following experimental induction of placentitis

Shavahn C. Loux, Claudia B. Fernandes, Pouya Dini, Kai Wang, Xiaogang Wu, David Baxter, Kirsten E. Scoggin, Mats H.T. Troedsson, Edward L. Squires, Barry A. Ball

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Intrauterine infection and inflammation remain a major cause of preterm labour in women and mares, with little known about small RNA (sRNA) expression in tissue or circulation. To better characterise placental inflammation (placentitis), we examined sRNA expression in the endometrium, chorioallantois and serum of mares with and without placentitis. Disease was induced in 10 mares via intracervical inoculation of Streptococcus equi ssp. zooepidemicus, either with moderate or high levels of inoculum; three uninoculated gestationally matched mares were used as controls. Matched chorioallantois and endometrium were sampled in two locations: Region 1, gross inflammation near cervical star with placental separation and Region 2, gross inflammation without placental separation. In Region 1, 26 sRNAs were altered in chorioallantois, while 20 were altered in endometrium. Within Region 2, changes were more subdued in both chorioallantois (10 sRNAs) and endometrium (two sRNAs). Within serum, we identified nine significantly altered sRNAs. In summary, we have characterised the expression of sRNA in the chorioallantois, the endometrium and the serum of mares with experimentally induced placentitis using next-generation sequencing, identifying significant changes within each tissue examined. These data should provide valuable information about the physiology of placental inflammation to clinicians and researchers alike.

Original languageEnglish
Pages (from-to)1144-1156
Number of pages13
JournalReproduction, fertility, and development
Issue number6
StatePublished - 2019

Bibliographical note

Funding Information:
This work was funded by the Kentucky Thoroughbred Association/Kentucky Thoroughbred Breeders and Owners, the American Quarter Horse Association Young Investigator Award, the Grayson-Jockey Club Research Foundation, the Albert Clay Endowment and the Paul Mellon post-doctoral fellowship. There are no competing financial interests to report.

Publisher Copyright:
© 2019 CSIRO.


  • infection
  • inflammation
  • microRNA
  • placenta
  • pregnancy

ASJC Scopus subject areas

  • Biotechnology
  • Reproductive Medicine
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology


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