SNAP-mediated protein–protein interactions essential for neurotransmitter release

W. M. De Bello; V. O’connor; T. Dresbach; S. W. Whiteheart; S. S.H. Wang; F. E. Schweizer; H. Bertz; J. E. Rothman; G. J. Schweizer

doi:10.1038/373626a0

SNAP-mediated protein–protein interactions essential for neurotransmitter release

W. M. De Bello, V. O’connor, T. Dresbach, S. W. Whiteheart, S. S.H. Wang, F. E. Schweizer, H. Bertz, J. E. Rothman, G. J. Schweizer

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

137 Scopus citations

Abstract

THE constitutive fusion of transport vesicles with intracellular membranes requires soluble proteins called SNAPs¹. Certain pre-synaptic proteins^2–1 implicated in synaptic vesicle exocytosis⁵ also bind SNAPs, suggesting that SNAPs participate in the calcium-regulated membrane fusion events mediating neurotransmitter release^{6, 7}. Here we show that injection of recombinant SNAPs into the giant synapse of squid enhances transmitter release. Conversely, injection of peptides designed to mimic the sites at which SNAP interacts with its binding partners inhibits transmitter release downstream of synaptic vesicle docking. A SNAP-dependent protein complex must therefore mediate transmitter release, showing that transmitter release shares a common molecular mechanism with constitutive membrane fusion.

Original language	English
Pages (from-to)	626-630
Number of pages	5
Journal	Nature
Volume	373
Issue number	6515
DOIs	https://doi.org/10.1038/373626a0
State	Published - Feb 16 1995

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@article{16c0738ea484478aa4da77413763ae12,

title = "SNAP-mediated protein–protein interactions essential for neurotransmitter release",

abstract = "THE constitutive fusion of transport vesicles with intracellular membranes requires soluble proteins called SNAPs1. Certain pre-synaptic proteins2–1 implicated in synaptic vesicle exocytosis5 also bind SNAPs, suggesting that SNAPs participate in the calcium-regulated membrane fusion events mediating neurotransmitter release6, 7. Here we show that injection of recombinant SNAPs into the giant synapse of squid enhances transmitter release. Conversely, injection of peptides designed to mimic the sites at which SNAP interacts with its binding partners inhibits transmitter release downstream of synaptic vesicle docking. A SNAP-dependent protein complex must therefore mediate transmitter release, showing that transmitter release shares a common molecular mechanism with constitutive membrane fusion.",

author = "{De Bello}, {W. M.} and V. O{\textquoteright}connor and T. Dresbach and Whiteheart, {S. W.} and Wang, {S. S.H.} and Schweizer, {F. E.} and H. Bertz and Rothman, {J. E.} and Schweizer, {G. J.}",

year = "1995",

month = feb,

day = "16",

doi = "10.1038/373626a0",

language = "English",

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TY - JOUR

T1 - SNAP-mediated protein–protein interactions essential for neurotransmitter release

AU - De Bello, W. M.

AU - O’connor, V.

AU - Dresbach, T.

AU - Whiteheart, S. W.

AU - Wang, S. S.H.

AU - Schweizer, F. E.

AU - Bertz, H.

AU - Rothman, J. E.

AU - Schweizer, G. J.

PY - 1995/2/16

Y1 - 1995/2/16

N2 - THE constitutive fusion of transport vesicles with intracellular membranes requires soluble proteins called SNAPs1. Certain pre-synaptic proteins2–1 implicated in synaptic vesicle exocytosis5 also bind SNAPs, suggesting that SNAPs participate in the calcium-regulated membrane fusion events mediating neurotransmitter release6, 7. Here we show that injection of recombinant SNAPs into the giant synapse of squid enhances transmitter release. Conversely, injection of peptides designed to mimic the sites at which SNAP interacts with its binding partners inhibits transmitter release downstream of synaptic vesicle docking. A SNAP-dependent protein complex must therefore mediate transmitter release, showing that transmitter release shares a common molecular mechanism with constitutive membrane fusion.

AB - THE constitutive fusion of transport vesicles with intracellular membranes requires soluble proteins called SNAPs1. Certain pre-synaptic proteins2–1 implicated in synaptic vesicle exocytosis5 also bind SNAPs, suggesting that SNAPs participate in the calcium-regulated membrane fusion events mediating neurotransmitter release6, 7. Here we show that injection of recombinant SNAPs into the giant synapse of squid enhances transmitter release. Conversely, injection of peptides designed to mimic the sites at which SNAP interacts with its binding partners inhibits transmitter release downstream of synaptic vesicle docking. A SNAP-dependent protein complex must therefore mediate transmitter release, showing that transmitter release shares a common molecular mechanism with constitutive membrane fusion.

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JO - Nature

JF - Nature

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SNAP-mediated protein–protein interactions essential for neurotransmitter release

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