Solid-phase immunoradiometric assay for C-reactive protein using magnetisable cellulose particles

F. C. De Beer, M. B. Pepys

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

An immunoradiometric assay (IRMA) for C-reactive protein (CRP) was developed using magnetisable cellulose particles as the solid-phase support for anti-CRP antibodies. 125I-labelled immunopurified anti-CRP antibody was used to quantitate the amount of CRP taken up by the solid phase. Unbound label was easily and rapidly removed by decantation after sedimenting the particles on a magnet. The assay could detect 1 μg CRP/l and had a range of up to 10 mg/l with the portion of the standard curve between 10 μg/l and 2-3 mg/l being linear. Fifty samples per hour could be processed manually from serum to CRP result with an intra-assay CV of 5.2% and an inter-assay CV of 10.0%, based on 5 replicates of 5 samples with CRP levels between 2 mg/l and 180 mg/l run in 5 separate assays. Fifty clinical samples were assayed in parallel with a standard electroimmunoassay and yielded a linear correlation coefficient (r) of 0.975 and a slope of 0.98. With its single, brief incubation step including all reagents and its simple phase separation procedure the present method may be the assay of choice when precise measurement of CRP concentrations is required rapidly.

Original languageEnglish
Pages (from-to)299-308
Number of pages10
JournalJournal of Immunological Methods
Volume50
Issue number3
DOIs
StatePublished - May 14 1982

Keywords

  • C-reactive protein
  • immunoradiometric assay
  • magnetisable cellulose particles

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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