Solid phase radioimmunoassays for human C-reactive protein

B. Shine, F. C. de Beer, M. B. Pepys

Research output: Contribution to journalArticlepeer-review

248 Scopus citations


Two new, rapid and sensitive radioimmunoassays for human C-reactive protein (CRP) have been established using antiserum coupled to magnetizable cellulose particles, which facilitate phase separation. A single antibody method, using solid phase anti-CRP, provides a sensitivity of 50 μg/l with a 1-h incubation time and intra- and inter-assay coefficients of variation of 10%. A double antibody method, using fluid phase rabbit anti-CRP serum and solid phase sheep anti-rabbit IgG serum, provides a sensitivity of 3 μg/l with an overnight incubation and intra- and inter-assay coefficients of variation of 10%. Among 468 sera from normal adult volunteer blood donors the median CRP concentration was 800 μg/l, interquartile range 340-1700 μg/l and range 70-29,000 μ/l. Ninety percent of samples contained less than 3 mg/1 and 99% less than 10 mg/1. Low levels (14-650 μg/l) of CRP were detected both in amniotic fluids and in cerebrospinal fluids.

Original languageEnglish
Pages (from-to)13-23
Number of pages11
JournalClinica Chimica Acta
Issue number1
StatePublished - Nov 25 1981

Bibliographical note

Funding Information:
We wish to thank Drs. G. Forrest and C. Dawes and Mrs. Elaine Kelly for preparing solid phase-coupled antisera, Prof. J. Landon for his support, and Dr. T.D. Davis, Dr. B.D. Williams and Prof. E. Thompson for providing clinical samples. The work was supported in part by MRC Programme Grant G979/51 to MBP.

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical


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