TY - JOUR
T1 - Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) bind to a multi-SNAP receptor complex in Golgi membranes
AU - Whiteheart, S. W.
AU - Brunner, M.
AU - Wilson, D. W.
AU - Wiedmann, M.
AU - Rothman, J. E.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1992/6/15
Y1 - 1992/6/15
N2 - Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) are required for the binding of N-ethylmaleimide-sensitive fusion protein (NSF) to Golgi membranes and are, therefore, required for intra-Golgi transport. We report the existence of distinct α/β-SNAP and α-SNAP-binding sites in Golgi membranes that appear to be part of the same receptor complex. Cross-linking studies with α-SNAP demonstrate that an integral membrane protein of between 30-40 kDa is the α-SNAP binding component of the multi-SNAP receptor complex. These data suggest that SNAPs function by independently binding to a multiSNAP membrane-receptor complex, thereby activating them to serve as adaptors for the targeting of NSF.
AB - Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) are required for the binding of N-ethylmaleimide-sensitive fusion protein (NSF) to Golgi membranes and are, therefore, required for intra-Golgi transport. We report the existence of distinct α/β-SNAP and α-SNAP-binding sites in Golgi membranes that appear to be part of the same receptor complex. Cross-linking studies with α-SNAP demonstrate that an integral membrane protein of between 30-40 kDa is the α-SNAP binding component of the multi-SNAP receptor complex. These data suggest that SNAPs function by independently binding to a multiSNAP membrane-receptor complex, thereby activating them to serve as adaptors for the targeting of NSF.
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M3 - Article
C2 - 1601890
AN - SCOPUS:0026720008
SN - 0021-9258
VL - 267
SP - 12239
EP - 12243
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 17
ER -