Abstract
Soybean is a rich source of high-quality proteins and an excellent food source of bioactive peptides. A tripeptide, Leu–Ser–Trp (LSW), was previously identified from soybean as an angiotensin-converting enzyme inhibitory peptide. In the present work, we further studied its antioxidant and anti-inflammatory activities in human vascular endothelial cells (EA.hy926) and elucidated the mechanisms underlying these biological activities. In tumor necrosis factor alpha (TNFα)-stimulated EA.hy926 cells, LSW significantly inhibited oxidative stress, both reduced superoxide and malondialdehyde levels (p < 0.001), owing to its free-radical-scavenging ability. LSW treatment also mitigated the elevated protein expression of vascular adhesion molecule-1 (p < 0.001) and cyclooxygenase 2 (p < 0.01) via inhibition of NF-κB and p38/JNK signaling, respectively. Additionally, LSW also inhibited the endogenous formation of TNFα and attenuated the expression of its two receptors in EA.hy926 cells. Furthermore, LSW upregulated sirtuin-1 level, which partially contributed to its anti-inflammatory activity. These results demonstrate the multiple roles of LSW in ameliorating vascular endothelial oxidative stress and inflammatory responses, which support its uses as a nutraceutical or functional food ingredient for combating endothelial dysfunction and cardiovascular diseases.
Original language | English |
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Article number | 3372 |
Journal | Foods |
Volume | 11 |
Issue number | 21 |
DOIs | |
State | Published - Nov 2022 |
Bibliographical note
Publisher Copyright:© 2022 by the authors.
Keywords
- MAPK signaling
- NF-κB signaling
- bioactive peptides
- endothelial cells
- inflammation
- malondialdehyde
- sirtuin-1
- soybean
- superoxide
ASJC Scopus subject areas
- Food Science
- Microbiology
- Health(social science)
- Health Professions (miscellaneous)
- Plant Science