Species and tissue differences in the rate of dissociation of ouabain from (Na+ + K+)-ATPase

Thomas Tobin, Ruth Henderson, Amar K. Sen

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

The rates of the association and dissociation reactions of ouabain with (Na+ + K+)-ATPase (ATP phosphohydrolase EC 3.6.1.3) were compared. The enzymes were prepared from kidney, heart and brain tissues obtained from guinea pig, dog and cat. The rate of dissociation of ouabain from (Na+ + K+)-ATPase varied about 60-fold between different species. The dissociation rate also differs between tissues within a given species. The rate of association of ouabain with these enzyme preparations did nog differ appreciably. The results suggest that the species differences in sensitivity to the cardiac glycosides are principally related to the different rates of dissociation of the drug-enzyme complex.

Original languageEnglish
Pages (from-to)551-555
Number of pages5
JournalBBA - Biomembranes
Volume274
Issue number2
DOIs
StatePublished - Aug 9 1972

Bibliographical note

Funding Information:
This work was supported by grant No. MT2485 from the Medical Research Council of Canada.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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