Specific immunological detection of the (V+C)- fibronectin isoform

Nancy Burton-Wurster; Hao Chen; Rina Gendelman; Michael L. Jackson; Lucille F. Gagliardo; Da Nian Gu; Russell R. Zelko; George Lust; James N. MacLeod

doi:10.1016/S0945-053X(02)00033-1

Specific immunological detection of the (V+C)^- fibronectin isoform

Nancy Burton-Wurster, Hao Chen, Rina Gendelman, Michael L. Jackson, Lucille F. Gagliardo, Da Nian Gu, Russell R. Zelko, George Lust, James N. MacLeod

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The population of fibronectins in adult mammalian cartilage includes high levels of a cartilage-specific (V+C)^- isoform which lacks the V, III-15, and I-10 segments and thus contains a novel junction between protein segments III-14 and I-11. We report production of a monoclonal antibody specific for (V+C)^- fibronectin without cross-recognition of V⁺C⁺ and V^-C⁺ isoforms found in plasma and other tissues. Presentation of epitope to this antibody requires the III-14/I-11 junction, but the epitope itself extends beyond 14 amino acids immediately surrounding the junction site and involves a conformational change in III-14 and/or the N-terminal portion of I-11. The antibody, designated Mab 5D10 anti (V+C)^-, displays specificity for (V+C)^- fibronectin from multiple mammalian species including humans and utility in immunoblots, immunohistochemistry, and ELISA.

Original language	English
Pages (from-to)	393-398
Number of pages	6
Journal	Matrix Biology
Volume	21
Issue number	5
DOIs	https://doi.org/10.1016/S0945-053X(02)00033-1
State	Published - Aug 2002

Bibliographical note

Funding Information:
This work was supported by NIH grant AR44340 and the Arthritis Foundation. We acknowledge the invaluable assistance of Dr Judith Appleton in the production of the monoclonal antibody. We thank Ms Bevin Zimmerman and Ms Alina Jo Williams for excellent technical help. We are grateful to Dr Jean Schwarzbauer of Princeton University for gifts of FN1, DN1 and DN2 from which the FN3, DN3, and all other expression constructs were derived, and for Mab IC3 that is specific for all isoforms of rat fibronectin. The fibronectin null mouse embryo fibroblasts were generous gifts from Dr Dean Mosher at the University of Wisconsin, Madison. We appreciate the invaluable secretarial support of Ms Dorothy Scorelle.

Funding

This work was supported by NIH grant AR44340 and the Arthritis Foundation. We acknowledge the invaluable assistance of Dr Judith Appleton in the production of the monoclonal antibody. We thank Ms Bevin Zimmerman and Ms Alina Jo Williams for excellent technical help. We are grateful to Dr Jean Schwarzbauer of Princeton University for gifts of FN1, DN1 and DN2 from which the FN3, DN3, and all other expression constructs were derived, and for Mab IC3 that is specific for all isoforms of rat fibronectin. The fibronectin null mouse embryo fibroblasts were generous gifts from Dr Dean Mosher at the University of Wisconsin, Madison. We appreciate the invaluable secretarial support of Ms Dorothy Scorelle.

Funders	Funder number
National Institutes of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases	R01AR044340
Arthritis Foundation

Keywords

(V+C) fibronectin
Biomarker
Cartilage
Monoclonal antibody
Osteoarthritis
Synovial fluid

ASJC Scopus subject areas

Molecular Biology

Access to Document

10.1016/S0945-053X(02)00033-1

Cite this

@article{27176f90c075465382813ea8fe2be4ba,

title = "Specific immunological detection of the (V+C)- fibronectin isoform",

abstract = "The population of fibronectins in adult mammalian cartilage includes high levels of a cartilage-specific (V+C)- isoform which lacks the V, III-15, and I-10 segments and thus contains a novel junction between protein segments III-14 and I-11. We report production of a monoclonal antibody specific for (V+C)- fibronectin without cross-recognition of V+C+ and V-C+ isoforms found in plasma and other tissues. Presentation of epitope to this antibody requires the III-14/I-11 junction, but the epitope itself extends beyond 14 amino acids immediately surrounding the junction site and involves a conformational change in III-14 and/or the N-terminal portion of I-11. The antibody, designated Mab 5D10 anti (V+C)-, displays specificity for (V+C)- fibronectin from multiple mammalian species including humans and utility in immunoblots, immunohistochemistry, and ELISA.",

keywords = "(V+C) fibronectin, Biomarker, Cartilage, Monoclonal antibody, Osteoarthritis, Synovial fluid",

author = "Nancy Burton-Wurster and Hao Chen and Rina Gendelman and Jackson, {Michael L.} and Gagliardo, {Lucille F.} and Gu, {Da Nian} and Zelko, {Russell R.} and George Lust and MacLeod, {James N.}",

note = "Funding Information: This work was supported by NIH grant AR44340 and the Arthritis Foundation. We acknowledge the invaluable assistance of Dr Judith Appleton in the production of the monoclonal antibody. We thank Ms Bevin Zimmerman and Ms Alina Jo Williams for excellent technical help. We are grateful to Dr Jean Schwarzbauer of Princeton University for gifts of FN1, DN1 and DN2 from which the FN3, DN3, and all other expression constructs were derived, and for Mab IC3 that is specific for all isoforms of rat fibronectin. The fibronectin null mouse embryo fibroblasts were generous gifts from Dr Dean Mosher at the University of Wisconsin, Madison. We appreciate the invaluable secretarial support of Ms Dorothy Scorelle.",

year = "2002",

month = aug,

doi = "10.1016/S0945-053X(02)00033-1",

language = "English",

volume = "21",

pages = "393--398",

number = "5",

}

TY - JOUR

T1 - Specific immunological detection of the (V+C)- fibronectin isoform

AU - Burton-Wurster, Nancy

AU - Chen, Hao

AU - Gendelman, Rina

AU - Jackson, Michael L.

AU - Gagliardo, Lucille F.

AU - Gu, Da Nian

AU - Zelko, Russell R.

AU - Lust, George

AU - MacLeod, James N.

N1 - Funding Information: This work was supported by NIH grant AR44340 and the Arthritis Foundation. We acknowledge the invaluable assistance of Dr Judith Appleton in the production of the monoclonal antibody. We thank Ms Bevin Zimmerman and Ms Alina Jo Williams for excellent technical help. We are grateful to Dr Jean Schwarzbauer of Princeton University for gifts of FN1, DN1 and DN2 from which the FN3, DN3, and all other expression constructs were derived, and for Mab IC3 that is specific for all isoforms of rat fibronectin. The fibronectin null mouse embryo fibroblasts were generous gifts from Dr Dean Mosher at the University of Wisconsin, Madison. We appreciate the invaluable secretarial support of Ms Dorothy Scorelle.

PY - 2002/8

Y1 - 2002/8

N2 - The population of fibronectins in adult mammalian cartilage includes high levels of a cartilage-specific (V+C)- isoform which lacks the V, III-15, and I-10 segments and thus contains a novel junction between protein segments III-14 and I-11. We report production of a monoclonal antibody specific for (V+C)- fibronectin without cross-recognition of V+C+ and V-C+ isoforms found in plasma and other tissues. Presentation of epitope to this antibody requires the III-14/I-11 junction, but the epitope itself extends beyond 14 amino acids immediately surrounding the junction site and involves a conformational change in III-14 and/or the N-terminal portion of I-11. The antibody, designated Mab 5D10 anti (V+C)-, displays specificity for (V+C)- fibronectin from multiple mammalian species including humans and utility in immunoblots, immunohistochemistry, and ELISA.

AB - The population of fibronectins in adult mammalian cartilage includes high levels of a cartilage-specific (V+C)- isoform which lacks the V, III-15, and I-10 segments and thus contains a novel junction between protein segments III-14 and I-11. We report production of a monoclonal antibody specific for (V+C)- fibronectin without cross-recognition of V+C+ and V-C+ isoforms found in plasma and other tissues. Presentation of epitope to this antibody requires the III-14/I-11 junction, but the epitope itself extends beyond 14 amino acids immediately surrounding the junction site and involves a conformational change in III-14 and/or the N-terminal portion of I-11. The antibody, designated Mab 5D10 anti (V+C)-, displays specificity for (V+C)- fibronectin from multiple mammalian species including humans and utility in immunoblots, immunohistochemistry, and ELISA.

KW - (V+C) fibronectin

KW - Biomarker

KW - Cartilage

KW - Monoclonal antibody

KW - Osteoarthritis

KW - Synovial fluid

UR - http://www.scopus.com/inward/record.url?scp=0036701557&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036701557&partnerID=8YFLogxK

U2 - 10.1016/S0945-053X(02)00033-1

DO - 10.1016/S0945-053X(02)00033-1

M3 - Article

C2 - 12225804

AN - SCOPUS:0036701557

SN - 0945-053X

VL - 21

SP - 393

EP - 398

JO - Matrix Biology

JF - Matrix Biology

IS - 5

ER -