Specific receptor for Escherichia coli heat-stable enterotoxin (STa) may determine susceptibility of piglets to diarrheal disease

Abdul M. Saeed; R. McMillian; Victoria Huckelberry; R. Abernathy; R. N. Greenberg

doi:10.1111/j.1574-6968.1987.tb02152.x

Specific receptor for Escherichia coli heat-stable enterotoxin (ST_a) may determine susceptibility of piglets to diarrheal disease

Abdul M. Saeed
, R. McMillian
, Victoria Huckelberry
, R. Abernathy
, R. N. Greenberg

Internal Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We have demonstrated the existence of a receptor that is specific for the Escherichia coli heat-stable enterotoxin (ST_a) on piglet's small intestine. The binding of ST_a to this receptor seemed specific, saturable and reversible. The receptor was demonstrated on brush border cells and membranes prepared from all of the piglets (n = 24) that developed clinical diarrhea when challenged with purified ST_a enterotoxin at the dose of 100 μg/kg body weight. The receptor was not demonstrated on intestinal brush border cells and membrane prepared from piglets (n = 3) that were refractory to the experimental induction of diarrhea. The data suggest that binding of the ST_a to a specific receptor on the small intestine of piglets is a prerequisite to the diarrheal response triggered by this toxin.

Original language	English
Pages (from-to)	247-251
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	43
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-6968.1987.tb02152.x
State	Published - Sep 1987

Bibliographical note

Funding Information:
This work was supported by the Veteran's Administration Research Merit Grant 001. We thank Mrs. Sue Stevens for her excellent secretarial assistance.

Funding

This work was supported by the Veteran's Administration Research Merit Grant 001. We thank Mrs. Sue Stevens for her excellent secretarial assistance.

Funders	Funder number
Veteran's Administration Medical Center	001

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

(Piglet)
Diarrhea
Escherichia coli
Heat-stable enterotoxin
Receptor

ASJC Scopus subject areas

General Medicine

Access to Document

10.1111/j.1574-6968.1987.tb02152.x

Cite this

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title = "Specific receptor for Escherichia coli heat-stable enterotoxin (STa) may determine susceptibility of piglets to diarrheal disease",

abstract = "We have demonstrated the existence of a receptor that is specific for the Escherichia coli heat-stable enterotoxin (STa) on piglet's small intestine. The binding of STa to this receptor seemed specific, saturable and reversible. The receptor was demonstrated on brush border cells and membranes prepared from all of the piglets (n = 24) that developed clinical diarrhea when challenged with purified STa enterotoxin at the dose of 100 μg/kg body weight. The receptor was not demonstrated on intestinal brush border cells and membrane prepared from piglets (n = 3) that were refractory to the experimental induction of diarrhea. The data suggest that binding of the STa to a specific receptor on the small intestine of piglets is a prerequisite to the diarrheal response triggered by this toxin.",

keywords = "(Piglet), Diarrhea, Escherichia coli, Heat-stable enterotoxin, Receptor",

author = "Saeed, \{Abdul M.\} and R. McMillian and Victoria Huckelberry and R. Abernathy and Greenberg, \{R. N.\}",

note = "Funding Information: This work was supported by the Veteran's Administration Research Merit Grant 001. We thank Mrs. Sue Stevens for her excellent secretarial assistance.",

year = "1987",

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doi = "10.1111/j.1574-6968.1987.tb02152.x",

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T1 - Specific receptor for Escherichia coli heat-stable enterotoxin (STa) may determine susceptibility of piglets to diarrheal disease

AU - Saeed, Abdul M.

AU - McMillian, R.

AU - Huckelberry, Victoria

AU - Abernathy, R.

AU - Greenberg, R. N.

N1 - Funding Information: This work was supported by the Veteran's Administration Research Merit Grant 001. We thank Mrs. Sue Stevens for her excellent secretarial assistance.

PY - 1987/9

Y1 - 1987/9

N2 - We have demonstrated the existence of a receptor that is specific for the Escherichia coli heat-stable enterotoxin (STa) on piglet's small intestine. The binding of STa to this receptor seemed specific, saturable and reversible. The receptor was demonstrated on brush border cells and membranes prepared from all of the piglets (n = 24) that developed clinical diarrhea when challenged with purified STa enterotoxin at the dose of 100 μg/kg body weight. The receptor was not demonstrated on intestinal brush border cells and membrane prepared from piglets (n = 3) that were refractory to the experimental induction of diarrhea. The data suggest that binding of the STa to a specific receptor on the small intestine of piglets is a prerequisite to the diarrheal response triggered by this toxin.

AB - We have demonstrated the existence of a receptor that is specific for the Escherichia coli heat-stable enterotoxin (STa) on piglet's small intestine. The binding of STa to this receptor seemed specific, saturable and reversible. The receptor was demonstrated on brush border cells and membranes prepared from all of the piglets (n = 24) that developed clinical diarrhea when challenged with purified STa enterotoxin at the dose of 100 μg/kg body weight. The receptor was not demonstrated on intestinal brush border cells and membrane prepared from piglets (n = 3) that were refractory to the experimental induction of diarrhea. The data suggest that binding of the STa to a specific receptor on the small intestine of piglets is a prerequisite to the diarrheal response triggered by this toxin.

KW - (Piglet)

KW - Diarrhea

KW - Escherichia coli

KW - Heat-stable enterotoxin

KW - Receptor

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