Specificity of the rat vesicular acetylcholine transporter

Myung Hee Kim, Mei Lu, Gary Rogers, Stanley Parsons, Louis B. Hersh

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The protein kinase A-deficient PC12 cell line PC12A123.7 lacks both choline acetyltransferase and the vesicular acetylcholine transporter. This cell line has been used to establish a stably transfected cell line expressing recombinant rat vesicular acetylcholine transporter that is appropriately trafficked to small synaptic vesicles. Acetylcholine is transported by the rat vesicular acetylcholine transporter at a maximal rate of 1.45 nmol acetylcholine/min/mg protein and exhibits a Km for transport of 2.5 mM. The transporter binds vesamicol with a Kd of 7.5 nM. The ability of structural analogs of acetylcholine to inhibit both acetylcholine uptake and vesamicol binding was measured. The results demonstrate that like Torpedo vesicular acetylcholine transporter, the mammalian transporter can bind a diverse group of acetylcholine analogs.

Original languageEnglish
Pages (from-to)473-476
Number of pages4
JournalNeurochemical Research
Issue number3-4
StatePublished - Apr 1 2003

Bibliographical note

Funding Information:
This research was supported in part by grants from the National Institute on Aging, AG05893 and AG05144.


  • Acetylcholine analogs
  • Acetylcholine transport
  • Inhibition
  • Kinetics
  • Vesamicol binding
  • Vesicular acetylcholine transporter

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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