Splanchnic extraction of phenylalanine in mature mares was not affected by threonine supplementation

S. L. Mastellar; T. Barnes; K. Cybulak; K. L. Urschel

doi:10.1016/j.tvjl.2015.10.009

Splanchnic extraction of phenylalanine in mature mares was not affected by threonine supplementation

S. L. Mastellar, T. Barnes, K. Cybulak, K. L. Urschel

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

This study determined splanchnic extraction of phenylalanine at two intakes of threonine. Six Thoroughbred mares were supplemented with isonitrogenous amounts of either threonine or glutamate. Dietary threonine intakes were 119 (+Thr) and 58 (Basal) mg/kg/day, respectively. Each horse received each diet twice and each was studied once with an oral and once with an intravenous (IV) infusion of [1-¹³C]phenylalanine. A 2-h primed, constant IV infusion of [¹³C]sodium bicarbonate and a 4-h primed, constant infusion of [1-¹³C]phenylalanine, either orally or IV, were used to measure isotopic enrichments. Phenylalanine kinetics were not affected by diet (P > 0.05). Values for the splanchnic extraction of phenylalanine were 26 ± 5% and 27 ± 3% for the +Thr and Basal supplemented diets, respectively. These values will improve the accuracy of future equine indicator amino acid oxidation studies.

Original language	English
Pages (from-to)	190-192
Number of pages	3
Journal	Veterinary Journal
Volume	207
DOIs	https://doi.org/10.1016/j.tvjl.2015.10.009
State	Published - Jan 1 2016

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Ltd.

Funding

The information reported in this paper is part of a project of the Kentucky Agricultural Experiment Station (15-07-010) and is published with the approval of the Director. This project was supported by Agriculture and Food Research Initiative Competitive Grant No. 2012-67015-19448 from the USDA National Institute of Food and Agriculture. Preliminary results of this project were presented as an abstract at the 2014 Joint Annual Meeting in Kansas City, MO, USA.

Funders	Funder number
National Institute of Food and Agriculture

Keywords

Amino acids
Horse
Phenylalanine kinetics
Splanchnic extraction

ASJC Scopus subject areas

Animal Science and Zoology
General Veterinary

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title = "Splanchnic extraction of phenylalanine in mature mares was not affected by threonine supplementation",

abstract = "This study determined splanchnic extraction of phenylalanine at two intakes of threonine. Six Thoroughbred mares were supplemented with isonitrogenous amounts of either threonine or glutamate. Dietary threonine intakes were 119 (+Thr) and 58 (Basal) mg/kg/day, respectively. Each horse received each diet twice and each was studied once with an oral and once with an intravenous (IV) infusion of [1-13C]phenylalanine. A 2-h primed, constant IV infusion of [13C]sodium bicarbonate and a 4-h primed, constant infusion of [1-13C]phenylalanine, either orally or IV, were used to measure isotopic enrichments. Phenylalanine kinetics were not affected by diet (P > 0.05). Values for the splanchnic extraction of phenylalanine were 26 ± 5% and 27 ± 3% for the +Thr and Basal supplemented diets, respectively. These values will improve the accuracy of future equine indicator amino acid oxidation studies.",

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AU - Barnes, T.

AU - Cybulak, K.

AU - Urschel, K. L.

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N2 - This study determined splanchnic extraction of phenylalanine at two intakes of threonine. Six Thoroughbred mares were supplemented with isonitrogenous amounts of either threonine or glutamate. Dietary threonine intakes were 119 (+Thr) and 58 (Basal) mg/kg/day, respectively. Each horse received each diet twice and each was studied once with an oral and once with an intravenous (IV) infusion of [1-13C]phenylalanine. A 2-h primed, constant IV infusion of [13C]sodium bicarbonate and a 4-h primed, constant infusion of [1-13C]phenylalanine, either orally or IV, were used to measure isotopic enrichments. Phenylalanine kinetics were not affected by diet (P > 0.05). Values for the splanchnic extraction of phenylalanine were 26 ± 5% and 27 ± 3% for the +Thr and Basal supplemented diets, respectively. These values will improve the accuracy of future equine indicator amino acid oxidation studies.

AB - This study determined splanchnic extraction of phenylalanine at two intakes of threonine. Six Thoroughbred mares were supplemented with isonitrogenous amounts of either threonine or glutamate. Dietary threonine intakes were 119 (+Thr) and 58 (Basal) mg/kg/day, respectively. Each horse received each diet twice and each was studied once with an oral and once with an intravenous (IV) infusion of [1-13C]phenylalanine. A 2-h primed, constant IV infusion of [13C]sodium bicarbonate and a 4-h primed, constant infusion of [1-13C]phenylalanine, either orally or IV, were used to measure isotopic enrichments. Phenylalanine kinetics were not affected by diet (P > 0.05). Values for the splanchnic extraction of phenylalanine were 26 ± 5% and 27 ± 3% for the +Thr and Basal supplemented diets, respectively. These values will improve the accuracy of future equine indicator amino acid oxidation studies.

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KW - Phenylalanine kinetics

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Splanchnic extraction of phenylalanine in mature mares was not affected by threonine supplementation

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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