Splenic and peritoneal B-1 cells differ in terms of transcriptional and proliferative features that separate peritoneal B-1 from splenic B-2 cells

Gavin M. Fischer; Laura A. Solt; William D. Hastings; Kejian Yang; Rachel M. Gerstein; Barbara S. Nikolajczyk; Stephen H. Clarke; Thomas L. Rothstein

doi:10.1006/cimm.2001.1860

Splenic and peritoneal B-1 cells differ in terms of transcriptional and proliferative features that separate peritoneal B-1 from splenic B-2 cells

Gavin M. Fischer, Laura A. Solt, William D. Hastings, Kejian Yang, Rachel M. Gerstein, Barbara S. Nikolajczyk, Stephen H. Clarke, Thomas L. Rothstein

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

B-1 cells constitute a distinct B cell subset with characteristic phenotypic and functional features. B-1 cells are highly represented among peritoneal lymphocytes; substantial numbers of B-1 cells are also located within splenic tissue. Here a number of differences in transcription factor and gene expression were identified that separate peritoneal B-1 and splenic B-2 cells, and then splenic B-1 cells obtained from immunoglobulin transgenic mice were tested for these parameters. Splenic B-1 cells resembled splenic B-2 cells rather than peritoneal B-1 cells in terms of nuclear expression of DNA-binding STAT3, CREB, and PU.1, with respect to transcriptional activation of IL-10, and in the failure to enter cell cycle in response to PMA. Splenic B-1 cells (B-1S) appear to constitute a unique population of B-1 cells, which, while sharing with peritoneal B-1 cells (B-1P) certain phenotypic features, differ from them in transcription factor and gene expression and in signaling for cell cycle progression.

Original language	English
Pages (from-to)	62-71
Number of pages	10
Journal	Cellular Immunology
Volume	213
Issue number	1
DOIs	https://doi.org/10.1006/cimm.2001.1860
State	Published - Oct 10 2001

Bibliographical note

Funding Information:
1This work was supported by grants from The Charles H. Hood Foundation (R.M.G.) and the Arthritis Foundation (S.H.C.), by American Cancer Society Grant IRG72-001-24 (B.S.N.), and by USPHS Grants AI29576 (S.H.C.), AI43587 (S.H.C.), and AI29690 (T.L.R.), awarded by the National Institutes of Health.

Keywords

B lymphocytes
CD5
Cellular proliferation
Cytokines
Transcription factors

ASJC Scopus subject areas

Immunology

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title = "Splenic and peritoneal B-1 cells differ in terms of transcriptional and proliferative features that separate peritoneal B-1 from splenic B-2 cells",

abstract = "B-1 cells constitute a distinct B cell subset with characteristic phenotypic and functional features. B-1 cells are highly represented among peritoneal lymphocytes; substantial numbers of B-1 cells are also located within splenic tissue. Here a number of differences in transcription factor and gene expression were identified that separate peritoneal B-1 and splenic B-2 cells, and then splenic B-1 cells obtained from immunoglobulin transgenic mice were tested for these parameters. Splenic B-1 cells resembled splenic B-2 cells rather than peritoneal B-1 cells in terms of nuclear expression of DNA-binding STAT3, CREB, and PU.1, with respect to transcriptional activation of IL-10, and in the failure to enter cell cycle in response to PMA. Splenic B-1 cells (B-1S) appear to constitute a unique population of B-1 cells, which, while sharing with peritoneal B-1 cells (B-1P) certain phenotypic features, differ from them in transcription factor and gene expression and in signaling for cell cycle progression.",

keywords = "B lymphocytes, CD5, Cellular proliferation, Cytokines, Transcription factors",

author = "Fischer, {Gavin M.} and Solt, {Laura A.} and Hastings, {William D.} and Kejian Yang and Gerstein, {Rachel M.} and Nikolajczyk, {Barbara S.} and Clarke, {Stephen H.} and Rothstein, {Thomas L.}",

note = "Funding Information: 1This work was supported by grants from The Charles H. Hood Foundation (R.M.G.) and the Arthritis Foundation (S.H.C.), by American Cancer Society Grant IRG72-001-24 (B.S.N.), and by USPHS Grants AI29576 (S.H.C.), AI43587 (S.H.C.), and AI29690 (T.L.R.), awarded by the National Institutes of Health.",

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AU - Solt, Laura A.

AU - Hastings, William D.

AU - Yang, Kejian

AU - Gerstein, Rachel M.

AU - Nikolajczyk, Barbara S.

AU - Clarke, Stephen H.

AU - Rothstein, Thomas L.

N1 - Funding Information: 1This work was supported by grants from The Charles H. Hood Foundation (R.M.G.) and the Arthritis Foundation (S.H.C.), by American Cancer Society Grant IRG72-001-24 (B.S.N.), and by USPHS Grants AI29576 (S.H.C.), AI43587 (S.H.C.), and AI29690 (T.L.R.), awarded by the National Institutes of Health.

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N2 - B-1 cells constitute a distinct B cell subset with characteristic phenotypic and functional features. B-1 cells are highly represented among peritoneal lymphocytes; substantial numbers of B-1 cells are also located within splenic tissue. Here a number of differences in transcription factor and gene expression were identified that separate peritoneal B-1 and splenic B-2 cells, and then splenic B-1 cells obtained from immunoglobulin transgenic mice were tested for these parameters. Splenic B-1 cells resembled splenic B-2 cells rather than peritoneal B-1 cells in terms of nuclear expression of DNA-binding STAT3, CREB, and PU.1, with respect to transcriptional activation of IL-10, and in the failure to enter cell cycle in response to PMA. Splenic B-1 cells (B-1S) appear to constitute a unique population of B-1 cells, which, while sharing with peritoneal B-1 cells (B-1P) certain phenotypic features, differ from them in transcription factor and gene expression and in signaling for cell cycle progression.

AB - B-1 cells constitute a distinct B cell subset with characteristic phenotypic and functional features. B-1 cells are highly represented among peritoneal lymphocytes; substantial numbers of B-1 cells are also located within splenic tissue. Here a number of differences in transcription factor and gene expression were identified that separate peritoneal B-1 and splenic B-2 cells, and then splenic B-1 cells obtained from immunoglobulin transgenic mice were tested for these parameters. Splenic B-1 cells resembled splenic B-2 cells rather than peritoneal B-1 cells in terms of nuclear expression of DNA-binding STAT3, CREB, and PU.1, with respect to transcriptional activation of IL-10, and in the failure to enter cell cycle in response to PMA. Splenic B-1 cells (B-1S) appear to constitute a unique population of B-1 cells, which, while sharing with peritoneal B-1 cells (B-1P) certain phenotypic features, differ from them in transcription factor and gene expression and in signaling for cell cycle progression.

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KW - Cellular proliferation

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KW - Transcription factors

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Splenic and peritoneal B-1 cells differ in terms of transcriptional and proliferative features that separate peritoneal B-1 from splenic B-2 cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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