Splenic and peritoneal B-1 cells differ in terms of transcriptional and proliferative features that separate peritoneal B-1 from splenic B-2 cells

Gavin M. Fischer; Laura A. Solt; William D. Hastings; Kejian Yang; Rachel M. Gerstein; Barbara S. Nikolajczyk; Stephen H. Clarke; Thomas L. Rothstein

doi:10.1006/cimm.2001.1860

Splenic and peritoneal B-1 cells differ in terms of transcriptional and proliferative features that separate peritoneal B-1 from splenic B-2 cells

Gavin M. Fischer, Laura A. Solt, William D. Hastings, Kejian Yang, Rachel M. Gerstein, Barbara S. Nikolajczyk, Stephen H. Clarke, Thomas L. Rothstein

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

B-1 cells constitute a distinct B cell subset with characteristic phenotypic and functional features. B-1 cells are highly represented among peritoneal lymphocytes; substantial numbers of B-1 cells are also located within splenic tissue. Here a number of differences in transcription factor and gene expression were identified that separate peritoneal B-1 and splenic B-2 cells, and then splenic B-1 cells obtained from immunoglobulin transgenic mice were tested for these parameters. Splenic B-1 cells resembled splenic B-2 cells rather than peritoneal B-1 cells in terms of nuclear expression of DNA-binding STAT3, CREB, and PU.1, with respect to transcriptional activation of IL-10, and in the failure to enter cell cycle in response to PMA. Splenic B-1 cells (B-1S) appear to constitute a unique population of B-1 cells, which, while sharing with peritoneal B-1 cells (B-1P) certain phenotypic features, differ from them in transcription factor and gene expression and in signaling for cell cycle progression.

Original language	English
Pages (from-to)	62-71
Number of pages	10
Journal	Cellular Immunology
Volume	213
Issue number	1
DOIs	https://doi.org/10.1006/cimm.2001.1860
State	Published - Oct 10 2001

Bibliographical note

Funding Information:
1This work was supported by grants from The Charles H. Hood Foundation (R.M.G.) and the Arthritis Foundation (S.H.C.), by American Cancer Society Grant IRG72-001-24 (B.S.N.), and by USPHS Grants AI29576 (S.H.C.), AI43587 (S.H.C.), and AI29690 (T.L.R.), awarded by the National Institutes of Health.

Funding

1This work was supported by grants from The Charles H. Hood Foundation (R.M.G.) and the Arthritis Foundation (S.H.C.), by American Cancer Society Grant IRG72-001-24 (B.S.N.), and by USPHS Grants AI29576 (S.H.C.), AI43587 (S.H.C.), and AI29690 (T.L.R.), awarded by the National Institutes of Health.

Funders	Funder number
National Institutes of Health (NIH)
American Cancer Society	IRG72-001-24
National Institute of Allergy and Infectious Diseases	R01AI029690
Arthritis Foundation
Charles H. Hood Foundation
U.S. Public Health Service	AI43587, AI29576

Keywords

B lymphocytes
CD5
Cellular proliferation
Cytokines
Transcription factors

ASJC Scopus subject areas

Immunology

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10.1006/cimm.2001.1860

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title = "Splenic and peritoneal B-1 cells differ in terms of transcriptional and proliferative features that separate peritoneal B-1 from splenic B-2 cells",

abstract = "B-1 cells constitute a distinct B cell subset with characteristic phenotypic and functional features. B-1 cells are highly represented among peritoneal lymphocytes; substantial numbers of B-1 cells are also located within splenic tissue. Here a number of differences in transcription factor and gene expression were identified that separate peritoneal B-1 and splenic B-2 cells, and then splenic B-1 cells obtained from immunoglobulin transgenic mice were tested for these parameters. Splenic B-1 cells resembled splenic B-2 cells rather than peritoneal B-1 cells in terms of nuclear expression of DNA-binding STAT3, CREB, and PU.1, with respect to transcriptional activation of IL-10, and in the failure to enter cell cycle in response to PMA. Splenic B-1 cells (B-1S) appear to constitute a unique population of B-1 cells, which, while sharing with peritoneal B-1 cells (B-1P) certain phenotypic features, differ from them in transcription factor and gene expression and in signaling for cell cycle progression.",

keywords = "B lymphocytes, CD5, Cellular proliferation, Cytokines, Transcription factors",

author = "Fischer, {Gavin M.} and Solt, {Laura A.} and Hastings, {William D.} and Kejian Yang and Gerstein, {Rachel M.} and Nikolajczyk, {Barbara S.} and Clarke, {Stephen H.} and Rothstein, {Thomas L.}",

note = "Funding Information: 1This work was supported by grants from The Charles H. Hood Foundation (R.M.G.) and the Arthritis Foundation (S.H.C.), by American Cancer Society Grant IRG72-001-24 (B.S.N.), and by USPHS Grants AI29576 (S.H.C.), AI43587 (S.H.C.), and AI29690 (T.L.R.), awarded by the National Institutes of Health.",

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AU - Yang, Kejian

AU - Gerstein, Rachel M.

AU - Nikolajczyk, Barbara S.

AU - Clarke, Stephen H.

AU - Rothstein, Thomas L.

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N2 - B-1 cells constitute a distinct B cell subset with characteristic phenotypic and functional features. B-1 cells are highly represented among peritoneal lymphocytes; substantial numbers of B-1 cells are also located within splenic tissue. Here a number of differences in transcription factor and gene expression were identified that separate peritoneal B-1 and splenic B-2 cells, and then splenic B-1 cells obtained from immunoglobulin transgenic mice were tested for these parameters. Splenic B-1 cells resembled splenic B-2 cells rather than peritoneal B-1 cells in terms of nuclear expression of DNA-binding STAT3, CREB, and PU.1, with respect to transcriptional activation of IL-10, and in the failure to enter cell cycle in response to PMA. Splenic B-1 cells (B-1S) appear to constitute a unique population of B-1 cells, which, while sharing with peritoneal B-1 cells (B-1P) certain phenotypic features, differ from them in transcription factor and gene expression and in signaling for cell cycle progression.

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Splenic and peritoneal B-1 cells differ in terms of transcriptional and proliferative features that separate peritoneal B-1 from splenic B-2 cells

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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