Spore forming Actinobacterial diversity of Cholistan Desert Pakistan: Polyphasic taxonomy, antimicrobial potential and chemical profiling

Adeela Fatima; Usman Aftab; Khaled A. Shaaban; Jon S. Thorson; Imran Sajid

doi:10.1186/s12866-019-1414-x

Spore forming Actinobacterial diversity of Cholistan Desert Pakistan: Polyphasic taxonomy, antimicrobial potential and chemical profiling

Adeela Fatima, Usman Aftab, Khaled A. Shaaban, Jon S. Thorson, Imran Sajid

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Background: Actinobacteria are famous for the production of unique secondary metabolites that help in controlling the continuously emerging drug resistance all over the globe. This study aimed at the investigation of an extreme environment the Cholistan desert, located in southern Punjab, Pakistan, for actinobacterial diversity and their activity against methicillin resistant Staphylococcus aureus (MRSA). The Cholistan desert is a sub-tropical and arid ecosystem with harsh environment, limited rainfall and low humidity. The 20 soil and sand samples were collected from different locations in the desert and the actinobacterial strains were selectively isolated. The isolated strains were identified using a polyphasic taxonomic approach including morphological, biochemical, physiological characterization, scanning electron microscopy (SEM) and by 16S rRNA gene sequencing. Results: A total of 110 desert actinobacterial strains were recovered, which were found to be belonging to 3 different families of the order Actinomycetales, including the family Streptomycetaceae, family Pseudonocardiaceae and the family Micrococcaceae. The most frequently isolated genus was Streptomyces along with the genera Pseudonocardia and Arthrobacter. The isolated strains exhibited promising antimicrobial activity against methicillin resistant Staphylococcus aureus (MRSA) with zone of inhibition in the range of 9-32 mm in antimicrobial screening assays. The chemical profiling by thin layer chromatography, HPLC-UV/Vis and LC-MS analysis depicted the presence of different structural classes of antibiotics. Conclusion: The study revealed that Cholistan desert harbors immense actinobacterial diversity and most of the strains produce structurally diverse bioactive secondary metabolites, which are a promising source of novel antimicrobial drug candidates.

Original language	English
Article number	49
Journal	BMC Microbiology
Volume	19
Issue number	1
DOIs	https://doi.org/10.1186/s12866-019-1414-x
State	Published - Feb 22 2019

Bibliographical note

Funding Information:
This work was supported by the grant from Higher Education Commission (HEC) Pakistan (Grant No. PIN: 112–31652-2BM1–172, and HEC-NRPU Project 2121). This work was also supported by National Institutes of Health grant R24 OD21479 (JST), The University of Kentucky, College of Pharmacy and the National Center for Advancing Translational Sciences (UL1TR001998 and UL1TR000117). The grants PIN: 112–31652-2BM1–172, and HEC-NRPU Project 2121, provided the support in terms of student’s stipend and experimental design, travel cost for sample collection, research supplies, reagents, DNA sequencing cost, data analysis and writing the paper. Grants R24 OD21479 (JST), UL1TR001998 and UL1TR000117 supported, in part, personnel and reagents associated with chemical screening, analytical methods and data analysis.

Publisher Copyright:
© 2019 The Author(s).

Keywords

16S rRNA gene sequencing
Actinobacterial diversity
Cholistan desert
MRSA
Polyphasic taxonomy
Scanning electron microscopy (SEM)

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

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Cite this

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title = "Spore forming Actinobacterial diversity of Cholistan Desert Pakistan: Polyphasic taxonomy, antimicrobial potential and chemical profiling",

abstract = "Background: Actinobacteria are famous for the production of unique secondary metabolites that help in controlling the continuously emerging drug resistance all over the globe. This study aimed at the investigation of an extreme environment the Cholistan desert, located in southern Punjab, Pakistan, for actinobacterial diversity and their activity against methicillin resistant Staphylococcus aureus (MRSA). The Cholistan desert is a sub-tropical and arid ecosystem with harsh environment, limited rainfall and low humidity. The 20 soil and sand samples were collected from different locations in the desert and the actinobacterial strains were selectively isolated. The isolated strains were identified using a polyphasic taxonomic approach including morphological, biochemical, physiological characterization, scanning electron microscopy (SEM) and by 16S rRNA gene sequencing. Results: A total of 110 desert actinobacterial strains were recovered, which were found to be belonging to 3 different families of the order Actinomycetales, including the family Streptomycetaceae, family Pseudonocardiaceae and the family Micrococcaceae. The most frequently isolated genus was Streptomyces along with the genera Pseudonocardia and Arthrobacter. The isolated strains exhibited promising antimicrobial activity against methicillin resistant Staphylococcus aureus (MRSA) with zone of inhibition in the range of 9-32 mm in antimicrobial screening assays. The chemical profiling by thin layer chromatography, HPLC-UV/Vis and LC-MS analysis depicted the presence of different structural classes of antibiotics. Conclusion: The study revealed that Cholistan desert harbors immense actinobacterial diversity and most of the strains produce structurally diverse bioactive secondary metabolites, which are a promising source of novel antimicrobial drug candidates.",

keywords = "16S rRNA gene sequencing, Actinobacterial diversity, Cholistan desert, MRSA, Polyphasic taxonomy, Scanning electron microscopy (SEM)",

author = "Adeela Fatima and Usman Aftab and Shaaban, {Khaled A.} and Thorson, {Jon S.} and Imran Sajid",

note = "Funding Information: This work was supported by the grant from Higher Education Commission (HEC) Pakistan (Grant No. PIN: 112–31652-2BM1–172, and HEC-NRPU Project 2121). This work was also supported by National Institutes of Health grant R24 OD21479 (JST), The University of Kentucky, College of Pharmacy and the National Center for Advancing Translational Sciences (UL1TR001998 and UL1TR000117). The grants PIN: 112–31652-2BM1–172, and HEC-NRPU Project 2121, provided the support in terms of student{\textquoteright}s stipend and experimental design, travel cost for sample collection, research supplies, reagents, DNA sequencing cost, data analysis and writing the paper. Grants R24 OD21479 (JST), UL1TR001998 and UL1TR000117 supported, in part, personnel and reagents associated with chemical screening, analytical methods and data analysis. Publisher Copyright: {\textcopyright} 2019 The Author(s).",

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doi = "10.1186/s12866-019-1414-x",

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T1 - Spore forming Actinobacterial diversity of Cholistan Desert Pakistan

T2 - Polyphasic taxonomy, antimicrobial potential and chemical profiling

AU - Fatima, Adeela

AU - Aftab, Usman

AU - Shaaban, Khaled A.

AU - Thorson, Jon S.

AU - Sajid, Imran

N1 - Funding Information: This work was supported by the grant from Higher Education Commission (HEC) Pakistan (Grant No. PIN: 112–31652-2BM1–172, and HEC-NRPU Project 2121). This work was also supported by National Institutes of Health grant R24 OD21479 (JST), The University of Kentucky, College of Pharmacy and the National Center for Advancing Translational Sciences (UL1TR001998 and UL1TR000117). The grants PIN: 112–31652-2BM1–172, and HEC-NRPU Project 2121, provided the support in terms of student’s stipend and experimental design, travel cost for sample collection, research supplies, reagents, DNA sequencing cost, data analysis and writing the paper. Grants R24 OD21479 (JST), UL1TR001998 and UL1TR000117 supported, in part, personnel and reagents associated with chemical screening, analytical methods and data analysis. Publisher Copyright: © 2019 The Author(s).

PY - 2019/2/22

Y1 - 2019/2/22

N2 - Background: Actinobacteria are famous for the production of unique secondary metabolites that help in controlling the continuously emerging drug resistance all over the globe. This study aimed at the investigation of an extreme environment the Cholistan desert, located in southern Punjab, Pakistan, for actinobacterial diversity and their activity against methicillin resistant Staphylococcus aureus (MRSA). The Cholistan desert is a sub-tropical and arid ecosystem with harsh environment, limited rainfall and low humidity. The 20 soil and sand samples were collected from different locations in the desert and the actinobacterial strains were selectively isolated. The isolated strains were identified using a polyphasic taxonomic approach including morphological, biochemical, physiological characterization, scanning electron microscopy (SEM) and by 16S rRNA gene sequencing. Results: A total of 110 desert actinobacterial strains were recovered, which were found to be belonging to 3 different families of the order Actinomycetales, including the family Streptomycetaceae, family Pseudonocardiaceae and the family Micrococcaceae. The most frequently isolated genus was Streptomyces along with the genera Pseudonocardia and Arthrobacter. The isolated strains exhibited promising antimicrobial activity against methicillin resistant Staphylococcus aureus (MRSA) with zone of inhibition in the range of 9-32 mm in antimicrobial screening assays. The chemical profiling by thin layer chromatography, HPLC-UV/Vis and LC-MS analysis depicted the presence of different structural classes of antibiotics. Conclusion: The study revealed that Cholistan desert harbors immense actinobacterial diversity and most of the strains produce structurally diverse bioactive secondary metabolites, which are a promising source of novel antimicrobial drug candidates.

AB - Background: Actinobacteria are famous for the production of unique secondary metabolites that help in controlling the continuously emerging drug resistance all over the globe. This study aimed at the investigation of an extreme environment the Cholistan desert, located in southern Punjab, Pakistan, for actinobacterial diversity and their activity against methicillin resistant Staphylococcus aureus (MRSA). The Cholistan desert is a sub-tropical and arid ecosystem with harsh environment, limited rainfall and low humidity. The 20 soil and sand samples were collected from different locations in the desert and the actinobacterial strains were selectively isolated. The isolated strains were identified using a polyphasic taxonomic approach including morphological, biochemical, physiological characterization, scanning electron microscopy (SEM) and by 16S rRNA gene sequencing. Results: A total of 110 desert actinobacterial strains were recovered, which were found to be belonging to 3 different families of the order Actinomycetales, including the family Streptomycetaceae, family Pseudonocardiaceae and the family Micrococcaceae. The most frequently isolated genus was Streptomyces along with the genera Pseudonocardia and Arthrobacter. The isolated strains exhibited promising antimicrobial activity against methicillin resistant Staphylococcus aureus (MRSA) with zone of inhibition in the range of 9-32 mm in antimicrobial screening assays. The chemical profiling by thin layer chromatography, HPLC-UV/Vis and LC-MS analysis depicted the presence of different structural classes of antibiotics. Conclusion: The study revealed that Cholistan desert harbors immense actinobacterial diversity and most of the strains produce structurally diverse bioactive secondary metabolites, which are a promising source of novel antimicrobial drug candidates.

KW - 16S rRNA gene sequencing

KW - Actinobacterial diversity

KW - Cholistan desert

KW - MRSA

KW - Polyphasic taxonomy

KW - Scanning electron microscopy (SEM)

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JO - BMC Microbiology

JF - BMC Microbiology

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ER -

Spore forming Actinobacterial diversity of Cholistan Desert Pakistan: Polyphasic taxonomy, antimicrobial potential and chemical profiling

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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