SR-BI-mediated selective lipid uptake segregates apoA-I and apoA-II catabolism

Maria C. De Beer, Deneys R. Van Der Westhuyzen, Nathan L. Whitaker, Nancy R. Webb, Frederick C. De Beer

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


The HDL receptor scavenger receptor class B type I (SR-BI) binds HDL and mediates the selective uptake of cholesteryl ester. We previously showed that remnants, produced when human HDL2 is catabolized in mice overexpressing SR-BI, become incrementally smaller, ultimately consisting of small α-migrating particles, distinct from preβ HDL. When mixed with mouse plasma, some remnant particles rapidly increase in size by associating with HDL without the mediation of cholesteryl ester transfer protein, LCAT, or phospholipid transfer protein. Here, we show that processing of HDL2 by SR-BI-overexpressing mice resulted in the preferential loss of apolipoprotein A-II (apoA-II). Short-term processing generated two distinct, small α-migrating particles. One particle (8.0 nm diameter) contained apoA-I and apoA-II; the other particle (7.7 nm diameter) contained only apoA-I. With extensive SR-BI processing, only the 7.7 run particle remained. Only the 8.0 nm remnants were able to associate with HDL. Compared with HDL2, this remnant was more readily taken up by the liver than by the kidney. We conclude that SR-BI-generated HDL remnants consist of particles with or without apoA-II and that only those containing apoA-II associate with HDL in an enzyme-independent manner. Extensive SR-BI processing generates small apoA-II-depleted particles unable to reassociate with HDL and readily taken up by the liver. This represents a pathway by which apoA-I and apoA-II catabolism are segregated.

Original languageEnglish
Pages (from-to)2143-2150
Number of pages8
JournalJournal of Lipid Research
Issue number10
StatePublished - Oct 2005


  • Apolipoprotein A-I
  • Apolipoprotein A-II
  • High density lipoprotein
  • High density lipoprotein receptor
  • High density lipoprotein size
  • Lipoprotein metabolism
  • Lipoproteins
  • Remnant high density lipoprotein
  • Scavenger receptor class B type I

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology


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