Stability of the glutamate content of synaptosomes during their preparation

J. W. Geddes; J. D. Newstead; J. D. Wood

doi:10.1007/BF00966168

Stability of the glutamate content of synaptosomes during their preparation

J. W. Geddes, J. D. Newstead, J. D. Wood

Neuroscience

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4 Scopus citations

Abstract

The glutamate content of synaptosomal-enriched fractions is stable during the preparation and isolation of the organelles provided that the temperature of the preparation is maintained at 0-2°C and that inorganic ions are not added to the homogenizing and centrifugation media. Consequently, drug-induced in vivo changes in the glutamate content of nerve endings should be reflected by the corresponding changes in glutamate levels of synaptosomes prepared from the brain tissue.

Original language	English
Pages (from-to)	1107-1116
Number of pages	10
Journal	Neurochemical Research
Volume	5
Issue number	10
DOIs	https://doi.org/10.1007/BF00966168
State	Published - Oct 1980

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1007/BF00966168

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title = "Stability of the glutamate content of synaptosomes during their preparation",

abstract = "The glutamate content of synaptosomal-enriched fractions is stable during the preparation and isolation of the organelles provided that the temperature of the preparation is maintained at 0-2°C and that inorganic ions are not added to the homogenizing and centrifugation media. Consequently, drug-induced in vivo changes in the glutamate content of nerve endings should be reflected by the corresponding changes in glutamate levels of synaptosomes prepared from the brain tissue.",

author = "Geddes, {J. W.} and Newstead, {J. D.} and Wood, {J. D.}",

year = "1980",

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doi = "10.1007/BF00966168",

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T1 - Stability of the glutamate content of synaptosomes during their preparation

AU - Geddes, J. W.

AU - Newstead, J. D.

AU - Wood, J. D.

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Y1 - 1980/10

N2 - The glutamate content of synaptosomal-enriched fractions is stable during the preparation and isolation of the organelles provided that the temperature of the preparation is maintained at 0-2°C and that inorganic ions are not added to the homogenizing and centrifugation media. Consequently, drug-induced in vivo changes in the glutamate content of nerve endings should be reflected by the corresponding changes in glutamate levels of synaptosomes prepared from the brain tissue.

AB - The glutamate content of synaptosomal-enriched fractions is stable during the preparation and isolation of the organelles provided that the temperature of the preparation is maintained at 0-2°C and that inorganic ions are not added to the homogenizing and centrifugation media. Consequently, drug-induced in vivo changes in the glutamate content of nerve endings should be reflected by the corresponding changes in glutamate levels of synaptosomes prepared from the brain tissue.

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JO - Neurochemical Research

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IS - 10

ER -

Stability of the glutamate content of synaptosomes during their preparation

Abstract

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