Stable Isotope-Resolved Metabolomics by NMR

Penghui Lin; Andrew N. Lane; Teresa W.M. Fan

doi:10.1007/978-1-4939-9690-2_9

Stable Isotope-Resolved Metabolomics by NMR

Penghui Lin, Andrew N. Lane, Teresa W.M. Fan

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

11 Scopus citations

Abstract

Stable isotopes enable the tracing of atoms from precursors to products via metabolic transformations in situ and in crude extracts. NMR has some unique capabilities that are especially suited for metabolic analysis, including atom position-resolved isotope analysis and isotope editing. In this chapter we describe NMR-based analysis of positional isotopomer distribution using metabolic tracers.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	151-168
Number of pages	18
DOIs	https://doi.org/10.1007/978-1-4939-9690-2_9
State	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	2037
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
This work was supported in part by NIH grants 1U24DK097215-01A1, CA163223-01A1, 5P20GM121327 (COBRE) the Carmen L. Buck endowment (to ANL), and the Edith D. Gardner Chair in Cancer Research (TWMF).

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

Keywords

Isotopomers
Quantification
Spectral editing
Stable isotope-resolved metabolomics

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-9690-2_9

Cite this

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abstract = "Stable isotopes enable the tracing of atoms from precursors to products via metabolic transformations in situ and in crude extracts. NMR has some unique capabilities that are especially suited for metabolic analysis, including atom position-resolved isotope analysis and isotope editing. In this chapter we describe NMR-based analysis of positional isotopomer distribution using metabolic tracers.",

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Y1 - 2019

N2 - Stable isotopes enable the tracing of atoms from precursors to products via metabolic transformations in situ and in crude extracts. NMR has some unique capabilities that are especially suited for metabolic analysis, including atom position-resolved isotope analysis and isotope editing. In this chapter we describe NMR-based analysis of positional isotopomer distribution using metabolic tracers.

AB - Stable isotopes enable the tracing of atoms from precursors to products via metabolic transformations in situ and in crude extracts. NMR has some unique capabilities that are especially suited for metabolic analysis, including atom position-resolved isotope analysis and isotope editing. In this chapter we describe NMR-based analysis of positional isotopomer distribution using metabolic tracers.

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KW - Quantification

KW - Spectral editing

KW - Stable isotope-resolved metabolomics

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Stable Isotope-Resolved Metabolomics by NMR

Abstract

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Bibliographical note

Keywords

ASJC Scopus subject areas

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