Stable isotopes enable the tracing of atoms from precursors to products via metabolic transformations in situ and in crude extracts. NMR has some unique capabilities that are especially suited for metabolic analysis, including atom position-resolved isotope analysis and isotope editing. In this chapter we describe NMR-based analysis of positional isotopomer distribution using metabolic tracers.
|Title of host publication||Methods in Molecular Biology|
|Number of pages||18|
|State||Published - 2019|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
This work was supported in part by NIH grants 1U24DK097215-01A1, CA163223-01A1, 5P20GM121327 (COBRE) the Carmen L. Buck endowment (to ANL), and the Edith D. Gardner Chair in Cancer Research (TWMF).
© Springer Science+Business Media, LLC, part of Springer Nature 2019.
- Spectral editing
- Stable isotope-resolved metabolomics
ASJC Scopus subject areas
- Molecular Biology