Stable Isotope-Resolved Metabolomics by NMR

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

13 Scopus citations

Abstract

Stable isotopes enable the tracing of atoms from precursors to products via metabolic transformations in situ and in crude extracts. NMR has some unique capabilities that are especially suited for metabolic analysis, including atom position-resolved isotope analysis and isotope editing. In this chapter we describe NMR-based analysis of positional isotopomer distribution using metabolic tracers.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages151-168
Number of pages18
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume2037
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

Keywords

  • Isotopomers
  • Quantification
  • Spectral editing
  • Stable isotope-resolved metabolomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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