Standard operating procedures for biospecimen collection, processing, and storage: From the consortium for the study of chronic pancreatitis, diabetes, and pancreatic cancer

William E. Fisher, Zobeida Cruz-Monserrate, Amy L. McElhany, Gregory B. Lesinski, Phil A. Hart, Ria Ghosh, George Van Buren, Douglas S. Fishman, Jo Ann S. Rinaudo, Jose Serrano, Sudhir Srivastava, Thomas MacE, Mark Topazian, Ziding Feng, Dhiraj Yadav, Stephen J. Pandol, Steven J. Hughes, Robert Y. Liu, Emily Lu, Robert OrrDavid C. Whitcomb, Amer S. Abouhamze, Hanno Steen, Zachary M. Sellers, David M. Troendle, Aliye Uc, Mark E. Lowe, Darwin L. Conwell

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

High-quality and well-annotated biorepositories are needed to better understand the pathophysiology and biologic mechanisms of chronic pancreatitis (CP) and its consequences. We report a methodology for the development of a robust standard operating procedure (SOP) for a biorepository based on the experience of the clinical centers within the consortium to study Chronic Pancreatitis, Diabetes and Pancreas Cancer Clinical Centers (CPDPC), supported by the National Cancer Institute and the National Institute for Diabetes and Digestive and Kidney Diseases as a unique multidisciplinary model to study CP, diabetes, and pancreatic cancer in both children and adults. Standard operating procedures from the CPDPC centers were evaluated and consolidated. The literature was reviewed for standard biorepository operating procedures that facilitated downstream molecular analysis. The existing literature on biobanking practices was harmonized with the SOPs from the clinical centers to produce a biorepository for pancreatic research. This article reports the methods and basic principles behind the creation of SOPs to develop a biorepository for the CPDPC. These will serve as a guide for investigators developing biorepositories in pancreas research. Rigorous and meticulous adherence to standardized biospecimen collection will facilitate investigations to better understand the pathophysiology and biologic mechanisms of CP, diabetes, and pancreatic cancer.

Original languageEnglish
Pages (from-to)1213-1221
Number of pages9
JournalPancreas
Volume47
Issue number10
DOIs
StatePublished - Nov 1 2018

Bibliographical note

Funding Information:
Research reported in this publication was supported by the National Cancer Institute and National Institute of Diabetes and Digestive and Kidney Diseases under award numbers U01DK108326 (Baylor College of Medicine), U01DK108327 (Ohio State University), U01DK108328 (University of Texas MD Anderson Cancer Center), U01DK108288 (Mayo Clinic), U01DK108306 (University of Pittsburgh), U01DK108314 (Cedars-Sinai Medical Center), U01DK108320 (University Of Florida), U01DK108323 (Indiana University), U01DK108300 (Stanford University), and U01 DK108334 (University of Iowa). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Funding Information:
W ell-annotated and characterized human biospecimens are needed to identify potentially diagnostic, prognostic, and predictive biomarkers.1 Through the acquisition of a cohort of well-characterized patients and associated biospecimens (blood, urine, saliva, stool, pancreatic and duodenal juice, stools, and when feasible pancreatic tissue), the proposed clinical research network will provide the resources and collaborative opportunities necessary for achieving many of the research objectives identified by the Chronic Pancreatitis, Diabetes and Pancreas Cancer Clinical Centers (CPDPC). The CPDPC is supported by the National Cancer Institute (NCI) and the National Institute for Diabetes and Digestive and Kidney Diseases (NIDDK) as a unique multidisciplinary model to study chronic pancreatitis, diabetes, and pancreatic cancer in both children and adults. Differences in

Publisher Copyright:
© 2018 Wolters Kluwer Health, Inc. All rights reserved.

Keywords

  • biorepository
  • biospecimens
  • pancreas
  • standard operating procedures

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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