Standardisation of the quantitation of serum amyloid A protein (SAA) in human serum

Nicole L. Godenir, Mohammed S. Jeenah, Gerhard A. Coetzee, Deneys R. Van der Westhuyzen, Alistair F. Strachan, Frederick C. De Beer

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

An adequate method for standardising the quantitation of serum amyloid A protein (SSA) in human serum was developed. Acute phase high density lipoprotien3 (HDL3) was used as a standard. The concentration of the SAA in the standard was determined by the use of purified SAA. After protein determination, various concentrations of purified SAA were run on SDS-polyacylamide gel together with the HDL3 standard containing an unknown amount of SAA amongst the apolipoproteins. From the standard curve obtained by pyridine extraction (Coomassie blue colour yield at A605 nm) the concentration of SAA in the HDL3 standard was determined. An established immunoradiometric assay (IRMA) for SAA was standardised with the HDL3. SAA concentration in normal and acute phase sera were determined.

Original languageEnglish
Pages (from-to)217-225
Number of pages9
JournalJournal of Immunological Methods
Volume83
Issue number2
DOIs
StatePublished - Nov 7 1985

Keywords

  • immunoradiometric assay (IRMA)
  • serum amyloid A protein (SAA)
  • standardization

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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