STARCH-EXCESS4 is a laforin-like phosphoglucan phosphatase required for starch degradation in Arabidopsis thaliana

Oliver Kötting, Diana Santelia, Christoph Edner, Simona Eicke, Tina Marthaler, Matthew S. Gentry, Sylviane Comparot-Moss, Jychian Chen, Alison M. Smith, Martin Steup, Gerhard Ritte, Samuel C. Zeeman

Research output: Contribution to journalArticlepeer-review

192 Scopus citations

Abstract

Starch is the major storage carbohydrate in plants. It is comprised of glucans that form semicrystalline granules. Glucan phosphorylation is a prerequisite for normal starch breakdown, but phosphoglucan metabolism is not understood. A putative protein phosphatase encoded at the Starch Excess 4 (SEX4) locus of Arabidopsis thaliana was recently shown to be required for normal starch breakdown. Here, we show that SEX4 is a phosphoglucan phosphatase in vivo and define its role within the starch degradation pathway. SEX4 dephosphorylates both the starch granule surface and soluble phosphoglucans in vitro, and sex4 null mutants accumulate phosphorylated intermediates of starch breakdown. These compounds are linear α-1,4-glucans esterified with one or two phosphate groups. They are released from starch granules by the glucan hydrolases α-amylase and isoamylase. In vitro experiments show that the rate of starch granule degradation is increased upon simultaneous phosphorylation and dephosphorylation of starch. We propose that glucan phosphorylating enzymes and phosphoglucan phosphatases work in synergy with glucan hydrolases to mediate efficient starch catabolism.

Original languageEnglish
Pages (from-to)334-346
Number of pages13
JournalPlant Cell
Volume21
Issue number1
DOIs
StatePublished - Jan 2009

ASJC Scopus subject areas

  • Plant Science

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