Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency

N. G. Grubb; David W. Rudy; Stephen D. Hall

doi:10.1016/0378-4347(95)00517-X

Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency

N. G. Grubb, David W. Rudy, Stephen D. Hall

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

A rapid, sensitive method was developed for the quantification of the R- and S-enantiomers of ketoprofen and their acyl glucuronide conjugates in the plasma and dialysate of hemodialysis-dependent anephric patients. Unconjugated R- and S-ketoprofen plasma concentrations were determined directly by liquid chromatography using a S,S-Whelk-O1 chiral stationary phase. R- and S-Ketoprofen glucuronide for use as standards were resolved using a C₁₈ reversed-phase HPLC column with a mobile phase containing the ion-pair reagent tetrabutylammonium hydrogen sulfate. Plasma glucuronides, however, could not be directly quantified due to matrix interference. Therefore, the glucuronides were isolated using reversed-phase HPLC and quantified after alkaline hydrolysis using the S,S-Whelk-O1 chiral stationary phase column.

Original language	English
Pages (from-to)	237-244
Number of pages	8
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	678
Issue number	2
DOIs	https://doi.org/10.1016/0378-4347(95)00517-X
State	Published - Apr 12 1996

Bibliographical note

Funding Information:
This work was supportebdy US Public Health Service Grants AG0763 and DK37994 and GCRC Grant M01 RR00750. DWR was supported by a Faculty DevelopmenAt ward in Clinical Pharmacologfyro m the PRMAF.

Keywords

Enantiomer separation
Ketoprofen
Ketoprofen glucuronide

ASJC Scopus subject areas

General Chemistry

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10.1016/0378-4347(95)00517-X

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title = "Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency",

abstract = "A rapid, sensitive method was developed for the quantification of the R- and S-enantiomers of ketoprofen and their acyl glucuronide conjugates in the plasma and dialysate of hemodialysis-dependent anephric patients. Unconjugated R- and S-ketoprofen plasma concentrations were determined directly by liquid chromatography using a S,S-Whelk-O1 chiral stationary phase. R- and S-Ketoprofen glucuronide for use as standards were resolved using a C18 reversed-phase HPLC column with a mobile phase containing the ion-pair reagent tetrabutylammonium hydrogen sulfate. Plasma glucuronides, however, could not be directly quantified due to matrix interference. Therefore, the glucuronides were isolated using reversed-phase HPLC and quantified after alkaline hydrolysis using the S,S-Whelk-O1 chiral stationary phase column.",

keywords = "Enantiomer separation, Ketoprofen, Ketoprofen glucuronide",

author = "Grubb, {N. G.} and Rudy, {David W.} and Hall, {Stephen D.}",

note = "Funding Information: This work was supportebdy US Public Health Service Grants AG0763 and DK37994 and GCRC Grant M01 RR00750. DWR was supported by a Faculty DevelopmenAt ward in Clinical Pharmacologfyro m the PRMAF.",

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doi = "10.1016/0378-4347(95)00517-X",

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Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency. / Grubb, N. G.; Rudy, David W.; Hall, Stephen D.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 678, No. 2, 12.04.1996, p. 237-244.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency

AU - Grubb, N. G.

AU - Rudy, David W.

AU - Hall, Stephen D.

N1 - Funding Information: This work was supportebdy US Public Health Service Grants AG0763 and DK37994 and GCRC Grant M01 RR00750. DWR was supported by a Faculty DevelopmenAt ward in Clinical Pharmacologfyro m the PRMAF.

PY - 1996/4/12

Y1 - 1996/4/12

N2 - A rapid, sensitive method was developed for the quantification of the R- and S-enantiomers of ketoprofen and their acyl glucuronide conjugates in the plasma and dialysate of hemodialysis-dependent anephric patients. Unconjugated R- and S-ketoprofen plasma concentrations were determined directly by liquid chromatography using a S,S-Whelk-O1 chiral stationary phase. R- and S-Ketoprofen glucuronide for use as standards were resolved using a C18 reversed-phase HPLC column with a mobile phase containing the ion-pair reagent tetrabutylammonium hydrogen sulfate. Plasma glucuronides, however, could not be directly quantified due to matrix interference. Therefore, the glucuronides were isolated using reversed-phase HPLC and quantified after alkaline hydrolysis using the S,S-Whelk-O1 chiral stationary phase column.

AB - A rapid, sensitive method was developed for the quantification of the R- and S-enantiomers of ketoprofen and their acyl glucuronide conjugates in the plasma and dialysate of hemodialysis-dependent anephric patients. Unconjugated R- and S-ketoprofen plasma concentrations were determined directly by liquid chromatography using a S,S-Whelk-O1 chiral stationary phase. R- and S-Ketoprofen glucuronide for use as standards were resolved using a C18 reversed-phase HPLC column with a mobile phase containing the ion-pair reagent tetrabutylammonium hydrogen sulfate. Plasma glucuronides, however, could not be directly quantified due to matrix interference. Therefore, the glucuronides were isolated using reversed-phase HPLC and quantified after alkaline hydrolysis using the S,S-Whelk-O1 chiral stationary phase column.

KW - Enantiomer separation

KW - Ketoprofen

KW - Ketoprofen glucuronide

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Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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