Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency

N. G. Grubb; David W. Rudy; Stephen D. Hall

doi:10.1016/0378-4347(95)00517-X

Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency

N. G. Grubb, David W. Rudy, Stephen D. Hall

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

A rapid, sensitive method was developed for the quantification of the R- and S-enantiomers of ketoprofen and their acyl glucuronide conjugates in the plasma and dialysate of hemodialysis-dependent anephric patients. Unconjugated R- and S-ketoprofen plasma concentrations were determined directly by liquid chromatography using a S,S-Whelk-O1 chiral stationary phase. R- and S-Ketoprofen glucuronide for use as standards were resolved using a C₁₈ reversed-phase HPLC column with a mobile phase containing the ion-pair reagent tetrabutylammonium hydrogen sulfate. Plasma glucuronides, however, could not be directly quantified due to matrix interference. Therefore, the glucuronides were isolated using reversed-phase HPLC and quantified after alkaline hydrolysis using the S,S-Whelk-O1 chiral stationary phase column.

Original language	English
Pages (from-to)	237-244
Number of pages	8
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	678
Issue number	2
DOIs	https://doi.org/10.1016/0378-4347(95)00517-X
State	Published - Apr 12 1996

Bibliographical note

Funding Information:
This work was supportebdy US Public Health Service Grants AG0763 and DK37994 and GCRC Grant M01 RR00750. DWR was supported by a Faculty DevelopmenAt ward in Clinical Pharmacologfyro m the PRMAF.

Funding

This work was supportebdy US Public Health Service Grants AG0763 and DK37994 and GCRC Grant M01 RR00750. DWR was supported by a Faculty DevelopmenAt ward in Clinical Pharmacologfyro m the PRMAF.

Funders	Funder number
US Public Health Service	AG0763, M01 RR00750
National Institute of Diabetes and Digestive and Kidney Diseases	R01DK037994

Keywords

Enantiomer separation
Ketoprofen
Ketoprofen glucuronide

ASJC Scopus subject areas

General Chemistry

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10.1016/0378-4347(95)00517-X

Cite this

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title = "Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency",

abstract = "A rapid, sensitive method was developed for the quantification of the R- and S-enantiomers of ketoprofen and their acyl glucuronide conjugates in the plasma and dialysate of hemodialysis-dependent anephric patients. Unconjugated R- and S-ketoprofen plasma concentrations were determined directly by liquid chromatography using a S,S-Whelk-O1 chiral stationary phase. R- and S-Ketoprofen glucuronide for use as standards were resolved using a C18 reversed-phase HPLC column with a mobile phase containing the ion-pair reagent tetrabutylammonium hydrogen sulfate. Plasma glucuronides, however, could not be directly quantified due to matrix interference. Therefore, the glucuronides were isolated using reversed-phase HPLC and quantified after alkaline hydrolysis using the S,S-Whelk-O1 chiral stationary phase column.",

keywords = "Enantiomer separation, Ketoprofen, Ketoprofen glucuronide",

author = "Grubb, \{N. G.\} and Rudy, \{David W.\} and Hall, \{Stephen D.\}",

note = "Funding Information: This work was supportebdy US Public Health Service Grants AG0763 and DK37994 and GCRC Grant M01 RR00750. DWR was supported by a Faculty DevelopmenAt ward in Clinical Pharmacologfyro m the PRMAF.",

year = "1996",

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doi = "10.1016/0378-4347(95)00517-X",

language = "English",

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Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency. / Grubb, N. G.; Rudy, David W.; Hall, Stephen D.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 678, No. 2, 12.04.1996, p. 237-244.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency

AU - Grubb, N. G.

AU - Rudy, David W.

AU - Hall, Stephen D.

N1 - Funding Information: This work was supportebdy US Public Health Service Grants AG0763 and DK37994 and GCRC Grant M01 RR00750. DWR was supported by a Faculty DevelopmenAt ward in Clinical Pharmacologfyro m the PRMAF.

PY - 1996/4/12

Y1 - 1996/4/12

N2 - A rapid, sensitive method was developed for the quantification of the R- and S-enantiomers of ketoprofen and their acyl glucuronide conjugates in the plasma and dialysate of hemodialysis-dependent anephric patients. Unconjugated R- and S-ketoprofen plasma concentrations were determined directly by liquid chromatography using a S,S-Whelk-O1 chiral stationary phase. R- and S-Ketoprofen glucuronide for use as standards were resolved using a C18 reversed-phase HPLC column with a mobile phase containing the ion-pair reagent tetrabutylammonium hydrogen sulfate. Plasma glucuronides, however, could not be directly quantified due to matrix interference. Therefore, the glucuronides were isolated using reversed-phase HPLC and quantified after alkaline hydrolysis using the S,S-Whelk-O1 chiral stationary phase column.

AB - A rapid, sensitive method was developed for the quantification of the R- and S-enantiomers of ketoprofen and their acyl glucuronide conjugates in the plasma and dialysate of hemodialysis-dependent anephric patients. Unconjugated R- and S-ketoprofen plasma concentrations were determined directly by liquid chromatography using a S,S-Whelk-O1 chiral stationary phase. R- and S-Ketoprofen glucuronide for use as standards were resolved using a C18 reversed-phase HPLC column with a mobile phase containing the ion-pair reagent tetrabutylammonium hydrogen sulfate. Plasma glucuronides, however, could not be directly quantified due to matrix interference. Therefore, the glucuronides were isolated using reversed-phase HPLC and quantified after alkaline hydrolysis using the S,S-Whelk-O1 chiral stationary phase column.

KW - Enantiomer separation

KW - Ketoprofen

KW - Ketoprofen glucuronide

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Stereoselective high-performance liquid chromatographic analysis of ketoprofen and its acyl glucuronides in chronic renal insufficiency

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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