Sterol and sesquiterpenoid biosynthesis during a growth cycle of tobacco cell suspension cultures

Joseph Chappell, Carol Von Lanken, Urs Vögeli, Prashant Bhatt

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The accumulation and biosynthesis of sterols and fungal elicitor-inducible sesquiterpenoids by tobacco (Nicotiana tabacum) cell suspension cultures were examined as a function of a 10 day culture cycle. Sterols accumulated concomitantly with fresh weight gain. The rate of sterol biosynthesis, measured as the incorporation rate of [14C]acetate and [3H]mevalonate, was maximal when the cultures entered into their rapid phase of growth. Changes in squalene synthetase enzyme activity correlated more closely with the in vivo synthesis rate and accumulation of sterols than 3-hydroxy-3-methylglutaryl CoA reductase (HMGR) enzyme activity. Cell cultures entering into the rapid phase of growth also responded maximally to fungal elicitor as measured by the production of capsidiol, an extracellular sesquiterpenoid. However, the rate of sesquiterpenoid biosynthesis, measured as the incorporation rate of [14C]acetate and [3H]mevalonate, could not be correlated with elicitor-inducible HMGR or sesquiterpene cyclase enzyme activities, nor elicitor-suppressible squalene synthetase enzyme activity.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalPlant Cell Reports
Volume8
Issue number1
DOIs
StatePublished - May 1989

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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