TY - JOUR
T1 - Stimulation of gastrointestinal antibody to Shiga toxin by orogastric immunization in mice
AU - SUCKOW, MARK A.
AU - KEREN, DAVID F.
AU - BROWN, J. EDWARD
AU - KEUSCH, GERALD T.
PY - 1994/2
Y1 - 1994/2
N2 - Shiga toxin (ST) is a protein toxin of Shigella dysenteriae type 1, a causative agent of severe diarrhoea and dysentery. In this report we describe the gastrointestinal secretory antibody response of mice following orogastric immunization with ST. Gastrointestinal secretions were sampled by a gastrointestinal lavage technique weekly for 5 weeks after initial immunization. Assay of lavage samples by ELISA showed that mice vaccinated orogastrically with various doses of ST developed gastrointestinal antibody to ST in a dose‐dependent manner. Serum anti‐ST activity developed by 5 weeks after initial immunization. The ability of ST to act as a mucosal immune adjuvant was investigated by coadministration of ST and keyhole limpet haemocyanin. In contrast to cholera toxin, a potent adjuvant, ST did not demonstrate adjuvant activity. The mouse gastrointestinal lavage model could be useful for further analysis of the cellular basis of ST immunogenicity.
AB - Shiga toxin (ST) is a protein toxin of Shigella dysenteriae type 1, a causative agent of severe diarrhoea and dysentery. In this report we describe the gastrointestinal secretory antibody response of mice following orogastric immunization with ST. Gastrointestinal secretions were sampled by a gastrointestinal lavage technique weekly for 5 weeks after initial immunization. Assay of lavage samples by ELISA showed that mice vaccinated orogastrically with various doses of ST developed gastrointestinal antibody to ST in a dose‐dependent manner. Serum anti‐ST activity developed by 5 weeks after initial immunization. The ability of ST to act as a mucosal immune adjuvant was investigated by coadministration of ST and keyhole limpet haemocyanin. In contrast to cholera toxin, a potent adjuvant, ST did not demonstrate adjuvant activity. The mouse gastrointestinal lavage model could be useful for further analysis of the cellular basis of ST immunogenicity.
KW - Shiga
KW - Shigella dysenteriae.
KW - cholera toxin
KW - mice
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U2 - 10.1111/j.1440-1711.1994.tb03758.x
DO - 10.1111/j.1440-1711.1994.tb03758.x
M3 - Article
C2 - 8157288
AN - SCOPUS:0028156871
SN - 0818-9641
VL - 72
SP - 69
EP - 74
JO - Immunology and Cell Biology
JF - Immunology and Cell Biology
IS - 1
ER -
