Stimulation of gastrointestinal antibody to Shiga toxin by orogastric immunization in mice

MARK A. SUCKOW; DAVID F. KEREN; J. EDWARD BROWN; GERALD T. KEUSCH

doi:10.1111/j.1440-1711.1994.tb03758.x

Stimulation of gastrointestinal antibody to Shiga toxin by orogastric immunization in mice

MARK A. SUCKOW, DAVID F. KEREN, J. EDWARD BROWN, GERALD T. KEUSCH

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Shiga toxin (ST) is a protein toxin of Shigella dysenteriae type 1, a causative agent of severe diarrhoea and dysentery. In this report we describe the gastrointestinal secretory antibody response of mice following orogastric immunization with ST. Gastrointestinal secretions were sampled by a gastrointestinal lavage technique weekly for 5 weeks after initial immunization. Assay of lavage samples by ELISA showed that mice vaccinated orogastrically with various doses of ST developed gastrointestinal antibody to ST in a dose‐dependent manner. Serum anti‐ST activity developed by 5 weeks after initial immunization. The ability of ST to act as a mucosal immune adjuvant was investigated by coadministration of ST and keyhole limpet haemocyanin. In contrast to cholera toxin, a potent adjuvant, ST did not demonstrate adjuvant activity. The mouse gastrointestinal lavage model could be useful for further analysis of the cellular basis of ST immunogenicity.

Original language	English
Pages (from-to)	69-74
Number of pages	6
Journal	Immunology and Cell Biology
Volume	72
Issue number	1
DOIs	https://doi.org/10.1111/j.1440-1711.1994.tb03758.x
State	Published - Feb 1994

Keywords

cholera toxin
mice
Shiga
Shigella dysenteriae.

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/j.1440-1711.1994.tb03758.x

Cite this

@article{dea51294cff143e197bc6a1fa76b6fb4,

title = "Stimulation of gastrointestinal antibody to Shiga toxin by orogastric immunization in mice",

abstract = "Shiga toxin (ST) is a protein toxin of Shigella dysenteriae type 1, a causative agent of severe diarrhoea and dysentery. In this report we describe the gastrointestinal secretory antibody response of mice following orogastric immunization with ST. Gastrointestinal secretions were sampled by a gastrointestinal lavage technique weekly for 5 weeks after initial immunization. Assay of lavage samples by ELISA showed that mice vaccinated orogastrically with various doses of ST developed gastrointestinal antibody to ST in a dose‐dependent manner. Serum anti‐ST activity developed by 5 weeks after initial immunization. The ability of ST to act as a mucosal immune adjuvant was investigated by coadministration of ST and keyhole limpet haemocyanin. In contrast to cholera toxin, a potent adjuvant, ST did not demonstrate adjuvant activity. The mouse gastrointestinal lavage model could be useful for further analysis of the cellular basis of ST immunogenicity.",

keywords = "cholera toxin, mice, Shiga, Shigella dysenteriae.",

author = "SUCKOW, {MARK A.} and KEREN, {DAVID F.} and BROWN, {J. EDWARD} and KEUSCH, {GERALD T.}",

year = "1994",

month = feb,

doi = "10.1111/j.1440-1711.1994.tb03758.x",

language = "English",

volume = "72",

pages = "69--74",

number = "1",

}

TY - JOUR

T1 - Stimulation of gastrointestinal antibody to Shiga toxin by orogastric immunization in mice

AU - SUCKOW, MARK A.

AU - KEREN, DAVID F.

AU - BROWN, J. EDWARD

AU - KEUSCH, GERALD T.

PY - 1994/2

Y1 - 1994/2

N2 - Shiga toxin (ST) is a protein toxin of Shigella dysenteriae type 1, a causative agent of severe diarrhoea and dysentery. In this report we describe the gastrointestinal secretory antibody response of mice following orogastric immunization with ST. Gastrointestinal secretions were sampled by a gastrointestinal lavage technique weekly for 5 weeks after initial immunization. Assay of lavage samples by ELISA showed that mice vaccinated orogastrically with various doses of ST developed gastrointestinal antibody to ST in a dose‐dependent manner. Serum anti‐ST activity developed by 5 weeks after initial immunization. The ability of ST to act as a mucosal immune adjuvant was investigated by coadministration of ST and keyhole limpet haemocyanin. In contrast to cholera toxin, a potent adjuvant, ST did not demonstrate adjuvant activity. The mouse gastrointestinal lavage model could be useful for further analysis of the cellular basis of ST immunogenicity.

AB - Shiga toxin (ST) is a protein toxin of Shigella dysenteriae type 1, a causative agent of severe diarrhoea and dysentery. In this report we describe the gastrointestinal secretory antibody response of mice following orogastric immunization with ST. Gastrointestinal secretions were sampled by a gastrointestinal lavage technique weekly for 5 weeks after initial immunization. Assay of lavage samples by ELISA showed that mice vaccinated orogastrically with various doses of ST developed gastrointestinal antibody to ST in a dose‐dependent manner. Serum anti‐ST activity developed by 5 weeks after initial immunization. The ability of ST to act as a mucosal immune adjuvant was investigated by coadministration of ST and keyhole limpet haemocyanin. In contrast to cholera toxin, a potent adjuvant, ST did not demonstrate adjuvant activity. The mouse gastrointestinal lavage model could be useful for further analysis of the cellular basis of ST immunogenicity.

KW - cholera toxin

KW - mice

KW - Shiga

KW - Shigella dysenteriae.

UR - http://www.scopus.com/inward/record.url?scp=0028156871&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028156871&partnerID=8YFLogxK

U2 - 10.1111/j.1440-1711.1994.tb03758.x

DO - 10.1111/j.1440-1711.1994.tb03758.x

M3 - Article

C2 - 8157288

AN - SCOPUS:0028156871

VL - 72

SP - 69

EP - 74

IS - 1

ER -

Stimulation of gastrointestinal antibody to Shiga toxin by orogastric immunization in mice

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this