Abstract
Shiga toxin (ST) is a protein toxin of Shigella dysenteriae type 1, a causative agent of severe diarrhoea and dysentery. In this report we describe the gastrointestinal secretory antibody response of mice following orogastric immunization with ST. Gastrointestinal secretions were sampled by a gastrointestinal lavage technique weekly for 5 weeks after initial immunization. Assay of lavage samples by ELISA showed that mice vaccinated orogastrically with various doses of ST developed gastrointestinal antibody to ST in a dose‐dependent manner. Serum anti‐ST activity developed by 5 weeks after initial immunization. The ability of ST to act as a mucosal immune adjuvant was investigated by coadministration of ST and keyhole limpet haemocyanin. In contrast to cholera toxin, a potent adjuvant, ST did not demonstrate adjuvant activity. The mouse gastrointestinal lavage model could be useful for further analysis of the cellular basis of ST immunogenicity.
Original language | English |
---|---|
Pages (from-to) | 69-74 |
Number of pages | 6 |
Journal | Immunology and Cell Biology |
Volume | 72 |
Issue number | 1 |
DOIs | |
State | Published - Feb 1994 |
Keywords
- cholera toxin
- mice
- Shiga
- Shigella dysenteriae.
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Cell Biology