Stimulation of gastrointestinal antibody to Shiga toxin by orogastric immunization in mice

MARK A. SUCKOW, DAVID F. KEREN, J. EDWARD BROWN, GERALD T. KEUSCH

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Shiga toxin (ST) is a protein toxin of Shigella dysenteriae type 1, a causative agent of severe diarrhoea and dysentery. In this report we describe the gastrointestinal secretory antibody response of mice following orogastric immunization with ST. Gastrointestinal secretions were sampled by a gastrointestinal lavage technique weekly for 5 weeks after initial immunization. Assay of lavage samples by ELISA showed that mice vaccinated orogastrically with various doses of ST developed gastrointestinal antibody to ST in a dose‐dependent manner. Serum anti‐ST activity developed by 5 weeks after initial immunization. The ability of ST to act as a mucosal immune adjuvant was investigated by coadministration of ST and keyhole limpet haemocyanin. In contrast to cholera toxin, a potent adjuvant, ST did not demonstrate adjuvant activity. The mouse gastrointestinal lavage model could be useful for further analysis of the cellular basis of ST immunogenicity.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalImmunology and Cell Biology
Volume72
Issue number1
DOIs
StatePublished - Feb 1994

Keywords

  • cholera toxin
  • mice
  • Shiga
  • Shigella dysenteriae.

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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