Storage behavior and changes in concentrations of abscisic acid and gibberellins during dormancy break and germination in seeds of Phellodendron amurense var. wilsonii (Rutaceae)

Shun Ying Chen, Ching Te Chien, Jerry M. Baskin, Carol C. Baskin

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

The medicinal Asian plant genus Phellodendron is known to contain several very important compounds that have biological action. The main purpose of this study was to determine whether seeds of Phellodendron amurense var. wilsonii can be stored and to characterize their dormancy. Seeds of this taxon stored at -20 and -80 °C and in liquid nitrogen retained their high germinability, indicating that they have orthodox storage behavior. Intact seeds from freshly collected fruits were dormant and required 12 weeks of cold stratification at 4 °C for complete germination. Scarifying the seed coat was partially effective in breaking seed dormancy. Exogenous gibberellins (GA3, GA4 and GA4+7) promoted germination of scarified seeds, GA4 and GA4+7 being more effective than GA3. Fluridone, an abscisic acid (ABA) biosynthesis inhibitor, was efficient in breaking dormancy, but it was less effective than GA4 or GA4+7 alone. Paclobutrazol, a GA biosynthesis inhibitor, inhibited seed germination, and the inhibitory effect was reversed completely by GA4 and by GA4+7. ABA content of seeds subjected to cold stratification or to incubation at 35/10 °C, which enhanced seed germination, was reduced about four- to sixfold compared to that of fresh seeds. Higher concentrations of GA3, GA4 and GA7 were detected in nondormant seeds and in seeds with an emerged radicle than in fresh seeds. Present results seem to indicate that dormancy in P. amurense var. wilsonii seeds is imposed partially by the seed coat and partially by high ABA content. ABA content decreased and GA3, GA4 and GA7 content increased during germination.

Original languageEnglish
Pages (from-to)275-284
Number of pages10
JournalTree Physiology
Volume30
Issue number2
DOIs
StatePublished - Dec 2009

Keywords

  • Cold stratification
  • Fluridone
  • Paclobutrazol
  • Seed germination
  • Seed storage

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Fingerprint

Dive into the research topics of 'Storage behavior and changes in concentrations of abscisic acid and gibberellins during dormancy break and germination in seeds of Phellodendron amurense var. wilsonii (Rutaceae)'. Together they form a unique fingerprint.

Cite this