@inproceedings{a7d54271dc8e4d199c38df65b332e373,
title = "Streamlining cell biology workflows: Integrating suspension culture, cell lysis, protein extraction and nucleic acid extraction",
abstract = "We have developed a platform which enables the culture and subsequent analysis of cells in suspension through immunohistology and nucleic acid assays. Implementation of a bucket design and passive pumping described by Young, et al. [1] allows for culture of cells in suspension for several days as well as treatment with drugs, cytokines, and proteinbased assays. The device has the added functionality of enabling single-step extraction of nucleic acids directly from culture using paramagnetic nucleic acid-binding beads. Extraction of DNA and mRNA from the culture is comparable to that of commercially available kits requiring several wash steps.",
keywords = "Cancer biology, Cell culture, Multiple myeloma, Nucleic acid extraction",
author = "{De Groot}, {T. E.} and Casavant, {B. P.} and Veserat, {K. S.} and Strotman, {L. N.} and Berry, {S. M.} and Beebe, {D. J.}",
year = "2013",
language = "English",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
pages = "1812--1814",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}
