TY - JOUR
T1 - Stromal Cell-Derived Factor 1-Mediated CXCR4 Signaling in Rat and Human Cortical Neural Progenitor Cells
AU - Peng, Hui
AU - Huang, Yunlong
AU - Rose, Jeremy
AU - Erichsen, David
AU - Herek, Shelley
AU - Fujii, Nobutaka
AU - Tamamura, Hirokazu
AU - Zheng, Jialin
PY - 2004/4/1
Y1 - 2004/4/1
N2 - Stromal cell-derived factor 1 (SDF-1) and the chemokine receptor CXCR4 are highly expressed in the nervous system. Knockout studies have suggested that both SDF-1 and CXCR4 play essential roles in cerebellar, hippocampal, and neocortical neural cell migration during embryogenesis. To extend these observations, CXCR4 signaling events in rat and human neural progenitor cells (NPCs) were examined. Our results show that CXCR4 is expressed in abundance on rat and human NPCs. Moreover, SDF-1α induced increased NPCs levels of inositol 1,4,5-triphosphate, extracellular signal-regulated kinases 1/2, Akt, c-Jun N-terminal kinase, and intracellular calcium whereas it diminished cyclic adenosine monophosphate. Finally, SDF-1α can induce human NPC chemotaxis in vitro, suggesting that CXCR4 plays a functional role in NPC migration. Both T140, a CXCR4 antagonist, and pertussis toxin (PTX), an inactivator of G protein-coupled receptors, abrogated these events. Ultimately, this study suggested that SDF-1α can influence NPC function through CXCR4 and that CXCR4 is functional on NPC.
AB - Stromal cell-derived factor 1 (SDF-1) and the chemokine receptor CXCR4 are highly expressed in the nervous system. Knockout studies have suggested that both SDF-1 and CXCR4 play essential roles in cerebellar, hippocampal, and neocortical neural cell migration during embryogenesis. To extend these observations, CXCR4 signaling events in rat and human neural progenitor cells (NPCs) were examined. Our results show that CXCR4 is expressed in abundance on rat and human NPCs. Moreover, SDF-1α induced increased NPCs levels of inositol 1,4,5-triphosphate, extracellular signal-regulated kinases 1/2, Akt, c-Jun N-terminal kinase, and intracellular calcium whereas it diminished cyclic adenosine monophosphate. Finally, SDF-1α can induce human NPC chemotaxis in vitro, suggesting that CXCR4 plays a functional role in NPC migration. Both T140, a CXCR4 antagonist, and pertussis toxin (PTX), an inactivator of G protein-coupled receptors, abrogated these events. Ultimately, this study suggested that SDF-1α can influence NPC function through CXCR4 and that CXCR4 is functional on NPC.
KW - CXCR4
KW - Intracellular signaling
KW - Neural progenitor cell
KW - Neurogenesis
KW - SDF-1α
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U2 - 10.1002/jnr.20045
DO - 10.1002/jnr.20045
M3 - Article
C2 - 15048928
AN - SCOPUS:1842584928
SN - 0360-4012
VL - 76
SP - 35
EP - 50
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
IS - 1
ER -