@article{baec48a4813444c98f5192e4fa76f6ba,
title = "Structural and functional characterization of CalS11, a TDP-rhamnose 3′- O -methyltransferase involved in calicheamicin biosynthesis",
abstract = "Sugar methyltransferases (MTs) are an important class of tailoring enzymes that catalyze the transfer of a methyl group from S-adenosyl-l-methionine to sugar-based N-, C- and O-nucleophiles. While sugar N- and C-MTs involved in natural product biosynthesis have been found to act on sugar nucleotide substrates prior to a subsequent glycosyltransferase reaction, corresponding sugar O-methylation reactions studied thus far occur after the glycosyltransfer reaction. Herein we report the first in vitro characterization using 1H-13C-gHSQC with isotopically labeled substrates and the X-ray structure determination at 1.55 {\AA} resolution of the TDP-3′-O-rhamnose-methyltransferase CalS11 from Micromonospora echinospora. This study highlights a unique NMR-based methyltransferase assay, implicates CalS11 to be a metal- and general acid/base-dependent O-methyltransferase, and as a first crystal structure for a TDP-hexose-O- methyltransferase, presents a new template for mechanistic studies and/or engineering.",
author = "Shanteri Singh and Aram Chang and Helmich, {Kate E.} and Bingman, {Craig A.} and Wrobel, {Russell L.} and Beebe, {Emily T.} and Makino, {Shin Ichi} and Aceti, {David J.} and Kevin Dyer and Hura, {Greg L.} and Manjula Sunkara and Morris, {Andrew J.} and Phillips, {George N.} and Thorson, {Jon S.}",
year = "2013",
month = jul,
day = "19",
doi = "10.1021/cb400068k",
language = "English",
volume = "8",
pages = "1632--1639",
number = "7",
}