Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis

Shanteri Singh; Youngchang Kim; Fengbin Wang; Lance Bigelow; Michael Endres; Madan K. Kharel; Gyorgy Babnigg; Craig A. Bingman; Andrzej Joachimiak; Jon S. Thorson; George N. Phillips

doi:10.1002/prot.24844

Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis

Shanteri Singh, Youngchang Kim, Fengbin Wang, Lance Bigelow, Michael Endres, Madan K. Kharel, Gyorgy Babnigg, Craig A. Bingman, Andrzej Joachimiak, Jon S. Thorson, George N. Phillips

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

AT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic structurally distinguished by its unique aminodideoxypentose-containing disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic pathway for this unusual sugar derives from comparative genomics where AtmS13 has been suggested as the contributing sugar aminotransferase (SAT). Determination of the AtmS13 X-ray structure at 1.50-Å resolution reveals it as a member of the aspartate aminotransferase fold type I (AAT-I). Structural comparisons of AtmS13 with homologous SATs that act upon similar substrates implicate potential active site residues that contribute to distinctions in sugar C5 (hexose vs. pentose) and/or sugar C2 (deoxy vs. hydroxyl) substrate specificity.

Original language	English
Pages (from-to)	1547-1554
Number of pages	8
Journal	Proteins: Structure, Function and Bioinformatics
Volume	83
Issue number	8
DOIs	https://doi.org/10.1002/prot.24844
State	Published - Aug 1 2015

Bibliographical note

Publisher Copyright:
© 2015 Wiley Periodicals, Inc.

Keywords

Carbohydrate
Indolocarbazole
Natural product
Sugar nucleotide
Transamination
X-ray crystallography

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology

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10.1002/prot.24844

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Singh, S., Kim, Y., Wang, F., Bigelow, L., Endres, M., Kharel, M. K., Babnigg, G., Bingman, C. A., Joachimiak, A., Thorson, J. S., & Phillips, G. N. (2015). Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis. Proteins: Structure, Function and Bioinformatics, 83(8), 1547-1554. https://doi.org/10.1002/prot.24844

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title = "Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis",

abstract = "AT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic structurally distinguished by its unique aminodideoxypentose-containing disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic pathway for this unusual sugar derives from comparative genomics where AtmS13 has been suggested as the contributing sugar aminotransferase (SAT). Determination of the AtmS13 X-ray structure at 1.50-{\AA} resolution reveals it as a member of the aspartate aminotransferase fold type I (AAT-I). Structural comparisons of AtmS13 with homologous SATs that act upon similar substrates implicate potential active site residues that contribute to distinctions in sugar C5 (hexose vs. pentose) and/or sugar C2 (deoxy vs. hydroxyl) substrate specificity.",

keywords = "Carbohydrate, Indolocarbazole, Natural product, Sugar nucleotide, Transamination, X-ray crystallography",

author = "Shanteri Singh and Youngchang Kim and Fengbin Wang and Lance Bigelow and Michael Endres and Kharel, {Madan K.} and Gyorgy Babnigg and Bingman, {Craig A.} and Andrzej Joachimiak and Thorson, {Jon S.} and Phillips, {George N.}",

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year = "2015",

month = aug,

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doi = "10.1002/prot.24844",

language = "English",

volume = "83",

pages = "1547--1554",

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Singh, S, Kim, Y, Wang, F, Bigelow, L, Endres, M, Kharel, MK, Babnigg, G, Bingman, CA, Joachimiak, A, Thorson, JS & Phillips, GN 2015, 'Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis', Proteins: Structure, Function and Bioinformatics, vol. 83, no. 8, pp. 1547-1554. https://doi.org/10.1002/prot.24844

TY - JOUR

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AU - Singh, Shanteri

AU - Kim, Youngchang

AU - Wang, Fengbin

AU - Bigelow, Lance

AU - Endres, Michael

AU - Kharel, Madan K.

AU - Babnigg, Gyorgy

AU - Bingman, Craig A.

AU - Joachimiak, Andrzej

AU - Thorson, Jon S.

AU - Phillips, George N.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - AT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic structurally distinguished by its unique aminodideoxypentose-containing disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic pathway for this unusual sugar derives from comparative genomics where AtmS13 has been suggested as the contributing sugar aminotransferase (SAT). Determination of the AtmS13 X-ray structure at 1.50-Å resolution reveals it as a member of the aspartate aminotransferase fold type I (AAT-I). Structural comparisons of AtmS13 with homologous SATs that act upon similar substrates implicate potential active site residues that contribute to distinctions in sugar C5 (hexose vs. pentose) and/or sugar C2 (deoxy vs. hydroxyl) substrate specificity.

AB - AT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic structurally distinguished by its unique aminodideoxypentose-containing disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic pathway for this unusual sugar derives from comparative genomics where AtmS13 has been suggested as the contributing sugar aminotransferase (SAT). Determination of the AtmS13 X-ray structure at 1.50-Å resolution reveals it as a member of the aspartate aminotransferase fold type I (AAT-I). Structural comparisons of AtmS13 with homologous SATs that act upon similar substrates implicate potential active site residues that contribute to distinctions in sugar C5 (hexose vs. pentose) and/or sugar C2 (deoxy vs. hydroxyl) substrate specificity.

KW - Carbohydrate

KW - Indolocarbazole

KW - Natural product

KW - Sugar nucleotide

KW - Transamination

KW - X-ray crystallography

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Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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