Abstract
AT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic structurally distinguished by its unique aminodideoxypentose-containing disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic pathway for this unusual sugar derives from comparative genomics where AtmS13 has been suggested as the contributing sugar aminotransferase (SAT). Determination of the AtmS13 X-ray structure at 1.50-Å resolution reveals it as a member of the aspartate aminotransferase fold type I (AAT-I). Structural comparisons of AtmS13 with homologous SATs that act upon similar substrates implicate potential active site residues that contribute to distinctions in sugar C5 (hexose vs. pentose) and/or sugar C2 (deoxy vs. hydroxyl) substrate specificity.
Original language | English |
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Pages (from-to) | 1547-1554 |
Number of pages | 8 |
Journal | Proteins: Structure, Function and Bioinformatics |
Volume | 83 |
Issue number | 8 |
DOIs | |
State | Published - Aug 1 2015 |
Bibliographical note
Publisher Copyright:© 2015 Wiley Periodicals, Inc.
Keywords
- Carbohydrate
- Indolocarbazole
- Natural product
- Sugar nucleotide
- Transamination
- X-ray crystallography
ASJC Scopus subject areas
- Structural Biology
- Biochemistry
- Molecular Biology