AT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic structurally distinguished by its unique aminodideoxypentose-containing disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic pathway for this unusual sugar derives from comparative genomics where AtmS13 has been suggested as the contributing sugar aminotransferase (SAT). Determination of the AtmS13 X-ray structure at 1.50-Å resolution reveals it as a member of the aspartate aminotransferase fold type I (AAT-I). Structural comparisons of AtmS13 with homologous SATs that act upon similar substrates implicate potential active site residues that contribute to distinctions in sugar C5 (hexose vs. pentose) and/or sugar C2 (deoxy vs. hydroxyl) substrate specificity.
|Number of pages||8|
|Journal||Proteins: Structure, Function and Bioinformatics|
|State||Published - Aug 1 2015|
Bibliographical notePublisher Copyright:
© 2015 Wiley Periodicals, Inc.
- Natural product
- Sugar nucleotide
- X-ray crystallography
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology