The structures of the O-glycosyltransferase LanGT2 and the engineered, C-C bond-forming variant LanGT2S8Ac show how the replacement of a single loop can change the functionality of the enzyme. Crystal structures of the enzymes in complex with a nonhydrolyzable nucleotide-sugar analogue revealed that there is a conformational transition to create the binding sites for the aglycon substrate. This induced-fit transition was explored by molecular docking experiments with various aglycon substrates.
|Number of pages||5|
|Journal||Angewandte Chemie - International Edition|
|State||Published - Feb 23 2015|
Bibliographical notePublisher Copyright:
© 2015 Wiley-VCH Verlag GmbH & Co. KGaA.
- Enzyme engineering
- Friedel-Crafts alkylation
ASJC Scopus subject areas
- Chemistry (all)