TY - JOUR
T1 - Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus
AU - Ferrari, Michele
AU - Folli, Claudia
AU - Pincolini, Elisa
AU - Mcclintock, Timothy S.
AU - Rössle, Manfred
AU - Berni, Rodolfo
AU - Cianci, Michele
PY - 2012/8
Y1 - 2012/8
N2 - Crustacean crustacyanin proteins are linked to the production and modification of carapace colour, with direct implications for fitness and survival. Here, the structural and functional properties of the two recombinant crustacyanin subunits H1 and H2 from the American lobster Homarus americanus are reported. The two subunits are structurally highly similar to the corresponding natural apo crustacyanin CRTC and CRTA subunits from the European lobster H. gammarus. Reconstitution studies of the recombinant crustacyanin proteins H1 and H2 with astaxanthin reproduced the bathochromic shift of 85-95 nm typical of the natural crustacyanin subunits from H. gammarus in complex with astaxanthin. Moreover, correlations between the presence of crustacyanin genes in crustacean species and the resulting carapace colours with the spectral properties of the subunits in complex with astaxanthin confirmed this genotype-phenotype linkage.
AB - Crustacean crustacyanin proteins are linked to the production and modification of carapace colour, with direct implications for fitness and survival. Here, the structural and functional properties of the two recombinant crustacyanin subunits H1 and H2 from the American lobster Homarus americanus are reported. The two subunits are structurally highly similar to the corresponding natural apo crustacyanin CRTC and CRTA subunits from the European lobster H. gammarus. Reconstitution studies of the recombinant crustacyanin proteins H1 and H2 with astaxanthin reproduced the bathochromic shift of 85-95 nm typical of the natural crustacyanin subunits from H. gammarus in complex with astaxanthin. Moreover, correlations between the presence of crustacyanin genes in crustacean species and the resulting carapace colours with the spectral properties of the subunits in complex with astaxanthin confirmed this genotype-phenotype linkage.
KW - astaxanthin
KW - bathochromic shift
KW - colouration
KW - lobster carapace
KW - recombinant carotenoproteins
UR - https://www.scopus.com/pages/publications/84864871509
UR - https://www.scopus.com/pages/publications/84864871509#tab=citedBy
U2 - 10.1107/S1744309112026103
DO - 10.1107/S1744309112026103
M3 - Article
C2 - 22869108
AN - SCOPUS:84864871509
SN - 1744-3091
VL - 68
SP - 846
EP - 853
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 8
ER -