Structural mass spectrometry for metabolomics

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

Structural mass spectrometry (MS) is a chemical structure-revealing analytical technique that measures mass/charge ratio of ions. Biochemical analyses in the context of metabolomics-which seeks chemical descriptions of cellular, organismal, and even community biology-pose challenges distinct from past applications of MS. The scientific need for both far greater depth and breadth of metabolic analytes is manifested analytically in two general approaches: the "Biomarker" approach and the "Pathway" approach. In this chapter, the basic principles of mass spectrometry relevant to these two approaches to metabolomics are reviewed. The emphasis is on practical aspects of how experimental design interacts with instrument designs and capabilities. Both simulated theoretical and real-life analyses are used to illustrate the key concepts and their ramifications.

Original languageEnglish
Title of host publicationThe Handbook of Metabolomics
EditorsTeresa Whei-Mei Fan, Richard Higashi, Andrew Lane
Pages61-97
Number of pages37
DOIs
StatePublished - 2012

Publication series

NameMethods in Pharmacology and Toxicology
Volume17
ISSN (Print)1557-2153
ISSN (Electronic)1940-6053

Keywords

  • High resolution MS
  • Isotopologue analysis
  • Metabolic pathways
  • Stable isotope resolved metabolomics

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics (all)
  • Pharmacology (medical)

Fingerprint

Dive into the research topics of 'Structural mass spectrometry for metabolomics'. Together they form a unique fingerprint.

Cite this