Structure-Activity Relationships of Organofluorine Inhibitors of β-Amyloid Self-Assembly

Béla Török, Abha Sood, Seema Bag, Aditya Kulkarni, Dmitry Borkin, Elizabeth Lawler, Sujaya Dasgupta, Shainaz Landge, Mohammed Abid, Weihong Zhou, Michelle Foster, Harry Levine, Marianna Török

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


A broad group of structurally diverse small organofluorine compounds were synthesized and evaluated as inhibitors of β-amyloid (Aβ) self-assembly. The main goal was to generate a diverse library of compounds with the same functional group and to observe general structural features that characterize inhibitors of Aβ oligomer and fibril formation, ultimately identifying structures for further focused inhibitor design. The common structural motifs in these compounds are CF 3-C-OH and CF 3-C-NH groups that were proposed to be binding units in our previous studies. A broad range of potential small-molecule inhibitors were synthesized by combining various carbocyclic and heteroaromatic rings with an array of substituents, generating a total of 106 molecules. The compounds were tested by standard methods such as thioflavin-T fluorescence spectroscopy for monitoring fibril formation, biotinyl Aβ 1-42 single-site streptavidin-based assays for observing oligomer formation, and atomic force microscopy for morphological studies. These assays revealed a number of structures that show significant inhibition against either Aβ fibril or oligomer formation. A detailed analysis of the structure-activity relationship of anti-fibril and -oligomer properties is provided. These data present further experimental evidence for the distinct nature of fibril versus oligomer formation and indicate that the interaction of the Aβ peptide with chiral small molecules is not stereospecific in nature.

Original languageEnglish
Pages (from-to)910-919
Number of pages10
Issue number5
StatePublished - May 2012


  • Alzheimer's disease
  • Chiral inhibitors
  • Heterocycles
  • Organofluorine compounds
  • β-amyloid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Pharmacology, Toxicology and Pharmaceutics (all)
  • Organic Chemistry


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