Structure and specificity of a permissive bacterial C-prenyltransferase

Sherif I. Elshahawi; Hongnan Cao; Khaled A. Shaaban; Larissa V. Ponomareva; Thangaiah Subramanian; Mark L. Farman; H. Peter Spielmann; George N. Phillips; Jon S. Thorson; Shanteri Singh

doi:10.1038/nchembio.2285

Structure and specificity of a permissive bacterial C-prenyltransferase

Sherif I. Elshahawi, Hongnan Cao, Khaled A. Shaaban, Larissa V. Ponomareva, Thangaiah Subramanian, Mark L. Farman, H. Peter Spielmann, George N. Phillips, Jon S. Thorson, Shanteri Singh

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

This study highlights the biochemical and structural characterization of the L-tryptophan C6 C-prenyltransferase (C-PT) PriB from Streptomyces sp. RM-5-8. PriB was found to be uniquely permissive to a diverse array of prenyl donors and acceptors including daptomycin. Two additional PTs also produced novel prenylated daptomycins with improved antibacterial activities over the parent drug.

Original language	English
Pages (from-to)	366-368
Number of pages	3
Journal	Nature Chemical Biology
Volume	13
Issue number	4
DOIs	https://doi.org/10.1038/nchembio.2285
State	Published - Apr 1 2017

Bibliographical note

Funding Information:
This work was supported by NIH grants R37 AI52188 and R01 CA203257 (J.S.T.), U01 GM098248 (G.N.P.) and NCATS (UL1TR001998). Daptomycin (Cubicin) was generously provided by Merck. We are grateful to J. Rohr, S. Van Lanen and J. Chappell (College of Pharmacy, University of Kentucky) for helpful discussion and facilitating access to shared equipment and/or reagents. We thank the University of Kentucky Mass Spectrometry Facility for the HR-ESI-MS support. This research also used resources of the Advanced Photon Source, a US Department of Energy (DOE) Office of Science user facility operated by Argonne National Laboratory (DE-AC02-06CH11357). Use of the Lilly Research Laboratories Collaborative Access Team (LRL-CAT) beamline at Sector 31 of the Advanced Photon Source was provided by Eli Lilly and Company.

Publisher Copyright:
© 2017 Nature America, Inc., part of Springer Nature. All rights reserved.

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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Structure and specificity of a permissive bacterial C-prenyltransferase

Abstract

Bibliographical note

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