Structure-based enhancement of the first anomeric glucokinase

Jie Yang, Lesley Liu, Jon S. Thorson

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


Promoting promiscuity. The availability of D-glucose-1-phosphate and its derivatives directly contributes to the efficiency of in vitro and in vivo glycosylation methodologies, such as natural-product glycorandomization. While one-step enzymatic routes to this vital class of sugar phosphates provide an attractive alternative to multistep chemical syntheses, no sugar kinase studied thus far is known to anomerically phosphorylate D-glucose. Herein we report both the discovery of inherent glucokinase (GlcK) activity in wild-type Lactococcus lactis galactokinase and the enhancement of this first GlcK to provide a catalyst capable of accepting a wide range of new monosaccharide substrates.

Original languageEnglish
Pages (from-to)992-996
Number of pages5
Issue number7
StatePublished - Jul 5 2004


  • Glucose
  • Kinases
  • Mutagenesis
  • Protein design
  • Sugars

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry


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