Structure-Based Enzyme Engineering and Its Impact on In Vitro Glycorandomization

Jon S. Thorson; William A. Barton; Dirk Hoffmeister; Christoph Albermann; Dimitar B. Nikolov

doi:10.1002/cbic.200300620

Structure-Based Enzyme Engineering and Its Impact on In Vitro Glycorandomization

Jon S. Thorson, William A. Barton, Dirk Hoffmeister, Christoph Albermann, Dimitar B. Nikolov

Research output: Contribution to journal › Review article › peer-review

76 Scopus citations

Abstract

Glycoengineering: This review highlights the application of structure-based enzyme engineering toward enhancing the promiscuity of nucleotidylytransferases (such as E_p, whose active site is shown here) and glycosyltransferases, which are essential to the in vitro glycorandomization (IVG) strategy. IVG is a versatile strategy with great potential for the generation of novel therapeutics as illustrated by the preparation of a library of diversified vancomycin analogues.

Original language	English
Pages (from-to)	16-25
Number of pages	10
Journal	ChemBioChem
Volume	5
Issue number	1
DOIs	https://doi.org/10.1002/cbic.200300620
State	Published - Jan 3 2004

Keywords

Antibiotics
Enzyme catalysis
Glycosyl phosphate
Natural products
Transferases

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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abstract = "Glycoengineering: This review highlights the application of structure-based enzyme engineering toward enhancing the promiscuity of nucleotidylytransferases (such as Ep, whose active site is shown here) and glycosyltransferases, which are essential to the in vitro glycorandomization (IVG) strategy. IVG is a versatile strategy with great potential for the generation of novel therapeutics as illustrated by the preparation of a library of diversified vancomycin analogues.",

keywords = "Antibiotics, Enzyme catalysis, Glycosyl phosphate, Natural products, Transferases",

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AU - Barton, William A.

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AU - Albermann, Christoph

AU - Nikolov, Dimitar B.

PY - 2004/1/3

Y1 - 2004/1/3

N2 - Glycoengineering: This review highlights the application of structure-based enzyme engineering toward enhancing the promiscuity of nucleotidylytransferases (such as Ep, whose active site is shown here) and glycosyltransferases, which are essential to the in vitro glycorandomization (IVG) strategy. IVG is a versatile strategy with great potential for the generation of novel therapeutics as illustrated by the preparation of a library of diversified vancomycin analogues.

AB - Glycoengineering: This review highlights the application of structure-based enzyme engineering toward enhancing the promiscuity of nucleotidylytransferases (such as Ep, whose active site is shown here) and glycosyltransferases, which are essential to the in vitro glycorandomization (IVG) strategy. IVG is a versatile strategy with great potential for the generation of novel therapeutics as illustrated by the preparation of a library of diversified vancomycin analogues.

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KW - Enzyme catalysis

KW - Glycosyl phosphate

KW - Natural products

KW - Transferases

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Structure-Based Enzyme Engineering and Its Impact on In Vitro Glycorandomization

Abstract

Keywords

ASJC Scopus subject areas

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