Structure-Based Enzyme Engineering and Its Impact on In Vitro Glycorandomization

Jon S. Thorson, William A. Barton, Dirk Hoffmeister, Christoph Albermann, Dimitar B. Nikolov

Research output: Contribution to journalReview articlepeer-review

75 Scopus citations

Abstract

Glycoengineering: This review highlights the application of structure-based enzyme engineering toward enhancing the promiscuity of nucleotidylytransferases (such as Ep, whose active site is shown here) and glycosyltransferases, which are essential to the in vitro glycorandomization (IVG) strategy. IVG is a versatile strategy with great potential for the generation of novel therapeutics as illustrated by the preparation of a library of diversified vancomycin analogues.

Original languageEnglish
Pages (from-to)16-25
Number of pages10
JournalChemBioChem
Volume5
Issue number1
DOIs
StatePublished - Jan 3 2004

Keywords

  • Antibiotics
  • Enzyme catalysis
  • Glycosyl phosphate
  • Natural products
  • Transferases

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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