Abstract
Some of the advances in nuclear magnetic resonance (NMR) that enable high information content analysis structure and quantitative analysis of metabolites and isotopomers, instead of providing a comprehensive account of metabolomics and metabonomics related NMR developments are studied. 1-D NMR is useful in metabolite profiling where compositional changes in a suite of metabolites are determined. It is useful to concentrate the extracts to be studied by susceptibility-matched volume limiting NMR tubes such as Shigemi tubes or small-volume probes such as a microprobe when dealing with samples of limited quantities such as biopsies or cell cultures, provided that the salt content is compatible. Although much of the NMR-based metabolomics research has been carried out in 1-D and 2-D, there are cases where 3-D experiments are called for, such as identification of metabolites which gather in a narrow spectral region.
| Original language | English |
|---|---|
| Pages (from-to) | 69-117 |
| Number of pages | 49 |
| Journal | Progress in Nuclear Magnetic Resonance Spectroscopy |
| Volume | 52 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Feb 4 2008 |
Bibliographical note
Funding Information:This work was supported in part by grants from the Susan G. Komen Foundation Grant BCTR0503648, National Cancer Institute 1 R01 CA101199-01, National Science Foundation/EPSCoR EPS-0447479, Kentucky Challenge for Excellence, Kentucky Science & Engineering Foundation KSEF-148-502-03-51, and the Brown Foundation of Kentucky. NIH Grant Number RR018733 from the National Center for Research Resources and the Brown Foundation of Kentucky.
Keywords
- 2-D NMR
- Isotopomer profiling
- Metabolic flux
- Metabolic pathways
- Metabolite profiling
- Stable isotopes
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Nuclear and High Energy Physics
- Spectroscopy