Structure-based profiling of metabolites and isotopomers by NMR

Teresa W.M. Fan; Andrew N. Lane

doi:10.1016/j.pnmrs.2007.03.002

Structure-based profiling of metabolites and isotopomers by NMR

Teresa W.M. Fan, Andrew N. Lane

Research output: Contribution to journal › Review article › peer-review

205 Scopus citations

Abstract

Some of the advances in nuclear magnetic resonance (NMR) that enable high information content analysis structure and quantitative analysis of metabolites and isotopomers, instead of providing a comprehensive account of metabolomics and metabonomics related NMR developments are studied. 1-D NMR is useful in metabolite profiling where compositional changes in a suite of metabolites are determined. It is useful to concentrate the extracts to be studied by susceptibility-matched volume limiting NMR tubes such as Shigemi tubes or small-volume probes such as a microprobe when dealing with samples of limited quantities such as biopsies or cell cultures, provided that the salt content is compatible. Although much of the NMR-based metabolomics research has been carried out in 1-D and 2-D, there are cases where 3-D experiments are called for, such as identification of metabolites which gather in a narrow spectral region.

Original language	English
Pages (from-to)	69-117
Number of pages	49
Journal	Progress in Nuclear Magnetic Resonance Spectroscopy
Volume	52
Issue number	2-3
DOIs	https://doi.org/10.1016/j.pnmrs.2007.03.002
State	Published - Feb 4 2008

Bibliographical note

Funding Information:
This work was supported in part by grants from the Susan G. Komen Foundation Grant BCTR0503648, National Cancer Institute 1 R01 CA101199-01, National Science Foundation/EPSCoR EPS-0447479, Kentucky Challenge for Excellence, Kentucky Science & Engineering Foundation KSEF-148-502-03-51, and the Brown Foundation of Kentucky. NIH Grant Number RR018733 from the National Center for Research Resources and the Brown Foundation of Kentucky.

Funding

This work was supported in part by grants from the Susan G. Komen Foundation Grant BCTR0503648, National Cancer Institute 1 R01 CA101199-01, National Science Foundation/EPSCoR EPS-0447479, Kentucky Challenge for Excellence, Kentucky Science & Engineering Foundation KSEF-148-502-03-51, and the Brown Foundation of Kentucky. NIH Grant Number RR018733 from the National Center for Research Resources and the Brown Foundation of Kentucky.

Funders	Funder number
Kentucky Challenge for Excellence
National Institutes of Health (NIH)	RR018733
National Childhood Cancer Registry – National Cancer Institute	EPS-0447479, 1 R01 CA101199-01
National Center for Research Resources
Susan G Komen Foundation	BCTR0503648
Kentucky Science and Engineering Foundation	KSEF-148-502-03-51

Keywords

2-D NMR
Isotopomer profiling
Metabolic flux
Metabolic pathways
Metabolite profiling
Stable isotopes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Nuclear and High Energy Physics
Spectroscopy

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10.1016/j.pnmrs.2007.03.002

Cite this

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title = "Structure-based profiling of metabolites and isotopomers by NMR",

abstract = "Some of the advances in nuclear magnetic resonance (NMR) that enable high information content analysis structure and quantitative analysis of metabolites and isotopomers, instead of providing a comprehensive account of metabolomics and metabonomics related NMR developments are studied. 1-D NMR is useful in metabolite profiling where compositional changes in a suite of metabolites are determined. It is useful to concentrate the extracts to be studied by susceptibility-matched volume limiting NMR tubes such as Shigemi tubes or small-volume probes such as a microprobe when dealing with samples of limited quantities such as biopsies or cell cultures, provided that the salt content is compatible. Although much of the NMR-based metabolomics research has been carried out in 1-D and 2-D, there are cases where 3-D experiments are called for, such as identification of metabolites which gather in a narrow spectral region.",

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author = "Fan, {Teresa W.M.} and Lane, {Andrew N.}",

note = "Funding Information: This work was supported in part by grants from the Susan G. Komen Foundation Grant BCTR0503648, National Cancer Institute 1 R01 CA101199-01, National Science Foundation/EPSCoR EPS-0447479, Kentucky Challenge for Excellence, Kentucky Science & Engineering Foundation KSEF-148-502-03-51, and the Brown Foundation of Kentucky. NIH Grant Number RR018733 from the National Center for Research Resources and the Brown Foundation of Kentucky. ",

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N2 - Some of the advances in nuclear magnetic resonance (NMR) that enable high information content analysis structure and quantitative analysis of metabolites and isotopomers, instead of providing a comprehensive account of metabolomics and metabonomics related NMR developments are studied. 1-D NMR is useful in metabolite profiling where compositional changes in a suite of metabolites are determined. It is useful to concentrate the extracts to be studied by susceptibility-matched volume limiting NMR tubes such as Shigemi tubes or small-volume probes such as a microprobe when dealing with samples of limited quantities such as biopsies or cell cultures, provided that the salt content is compatible. Although much of the NMR-based metabolomics research has been carried out in 1-D and 2-D, there are cases where 3-D experiments are called for, such as identification of metabolites which gather in a narrow spectral region.

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Structure-based profiling of metabolites and isotopomers by NMR

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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