Structure-based profiling of metabolites and isotopomers by NMR

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203 Scopus citations

Abstract

Some of the advances in nuclear magnetic resonance (NMR) that enable high information content analysis structure and quantitative analysis of metabolites and isotopomers, instead of providing a comprehensive account of metabolomics and metabonomics related NMR developments are studied. 1-D NMR is useful in metabolite profiling where compositional changes in a suite of metabolites are determined. It is useful to concentrate the extracts to be studied by susceptibility-matched volume limiting NMR tubes such as Shigemi tubes or small-volume probes such as a microprobe when dealing with samples of limited quantities such as biopsies or cell cultures, provided that the salt content is compatible. Although much of the NMR-based metabolomics research has been carried out in 1-D and 2-D, there are cases where 3-D experiments are called for, such as identification of metabolites which gather in a narrow spectral region.

Original languageEnglish
Pages (from-to)69-117
Number of pages49
JournalProgress in Nuclear Magnetic Resonance Spectroscopy
Volume52
Issue number2-3
DOIs
StatePublished - Feb 4 2008

Bibliographical note

Funding Information:
This work was supported in part by grants from the Susan G. Komen Foundation Grant BCTR0503648, National Cancer Institute 1 R01 CA101199-01, National Science Foundation/EPSCoR EPS-0447479, Kentucky Challenge for Excellence, Kentucky Science & Engineering Foundation KSEF-148-502-03-51, and the Brown Foundation of Kentucky. NIH Grant Number RR018733 from the National Center for Research Resources and the Brown Foundation of Kentucky.

Keywords

  • 2-D NMR
  • Isotopomer profiling
  • Metabolic flux
  • Metabolic pathways
  • Metabolite profiling
  • Stable isotopes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Nuclear and High Energy Physics
  • Spectroscopy

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