Structure of the cholera toxin secretion channel in its closed state

Steve L. Reichow; Konstantin V. Korotkov; Wim G.J. Hol; Tamir Gonen

doi:10.1038/nsmb.1910

Structure of the cholera toxin secretion channel in its closed state

Steve L. Reichow
, Konstantin V. Korotkov
, Wim G.J. Hol
, Tamir Gonen

Research output: Contribution to journal › Article › peer-review

121 Scopus citations

Abstract

The type II secretion system (T2SS) is a macromolecular complex spanning the inner and outer membranes of Gram-negative bacteria. Remarkably, the T2SS secretes folded proteins, including multimeric assemblies such as cholera toxin and heat-labile enterotoxin from Vibrio cholerae and enterotoxigenic Escherichia coli, respectively. The major outer membrane T2SS protein is the 'secretin' GspD. Cryo-EM reconstruction of the V. cholerae secretin at 19-Å resolution reveals a dodecameric structure reminiscent of a barrel, with a large channel at its center that contains a closed periplasmic gate. The GspD periplasmic domain forms a vestibule with a conserved constriction, and it binds to a pentameric exoprotein and to the trimeric tip of the T2SS pseudopilus. By combining our results with structures of the cholera toxin and T2SS pseudopilus tip, we provide a structural basis for a possible secretion mechanism of the T2SS.

Original language	English
Pages (from-to)	1226-1232
Number of pages	7
Journal	Nature Structural and Molecular Biology
Volume	17
Issue number	10
DOIs	https://doi.org/10.1038/nsmb.1910
State	Published - Oct 2010

Funding

We thank the Murdock Charitable Trust and the Washington Research Foundation for generous support of our cryo-EM facility. We are grateful to J. Sun, M. Gonen, B. Vollmar and S. Turley for contributions to the earlier stages of this work; M. Bagdasarian (Michigan State University) for a VcGspD-containing plasmid; and J. DelaRosa for assistance with protein preparation. We thank A. J. Merz for helpful discussions. We thank N. Korotkova and P. Wallace for discussion of SPR experiments. Part of this work was conducted at the University of Washington NanoTech User Facility, a member of the US National Science Foundation (NSF) National Nanotechnology Infrastructure Network (NNIN). This research is supported by the US National Institutes of Health grant AI34501. The Gonen laboratory is supported in part by the Howard Hughes Medical Institute Early Career Scientist program.

Funders	Funder number
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China
National Institutes of Health (NIH)
Howard Hughes Medical Institute
Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases	R01AI034501
M.J. Murdock Charitable Trust
Washington Research Foundation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Structural Biology
Molecular Biology

Access to Document

10.1038/nsmb.1910

Cite this

@article{d5cf2398956b49bf8d7b4aab94b83c54,

title = "Structure of the cholera toxin secretion channel in its closed state",

abstract = "The type II secretion system (T2SS) is a macromolecular complex spanning the inner and outer membranes of Gram-negative bacteria. Remarkably, the T2SS secretes folded proteins, including multimeric assemblies such as cholera toxin and heat-labile enterotoxin from Vibrio cholerae and enterotoxigenic Escherichia coli, respectively. The major outer membrane T2SS protein is the 'secretin' GspD. Cryo-EM reconstruction of the V. cholerae secretin at 19-{\AA} resolution reveals a dodecameric structure reminiscent of a barrel, with a large channel at its center that contains a closed periplasmic gate. The GspD periplasmic domain forms a vestibule with a conserved constriction, and it binds to a pentameric exoprotein and to the trimeric tip of the T2SS pseudopilus. By combining our results with structures of the cholera toxin and T2SS pseudopilus tip, we provide a structural basis for a possible secretion mechanism of the T2SS.",

author = "Reichow, \{Steve L.\} and Korotkov, \{Konstantin V.\} and Hol, \{Wim G.J.\} and Tamir Gonen",

year = "2010",

month = oct,

doi = "10.1038/nsmb.1910",

language = "English",

volume = "17",

pages = "1226--1232",

number = "10",

}

TY - JOUR

T1 - Structure of the cholera toxin secretion channel in its closed state

AU - Reichow, Steve L.

AU - Korotkov, Konstantin V.

AU - Hol, Wim G.J.

AU - Gonen, Tamir

PY - 2010/10

Y1 - 2010/10

N2 - The type II secretion system (T2SS) is a macromolecular complex spanning the inner and outer membranes of Gram-negative bacteria. Remarkably, the T2SS secretes folded proteins, including multimeric assemblies such as cholera toxin and heat-labile enterotoxin from Vibrio cholerae and enterotoxigenic Escherichia coli, respectively. The major outer membrane T2SS protein is the 'secretin' GspD. Cryo-EM reconstruction of the V. cholerae secretin at 19-Å resolution reveals a dodecameric structure reminiscent of a barrel, with a large channel at its center that contains a closed periplasmic gate. The GspD periplasmic domain forms a vestibule with a conserved constriction, and it binds to a pentameric exoprotein and to the trimeric tip of the T2SS pseudopilus. By combining our results with structures of the cholera toxin and T2SS pseudopilus tip, we provide a structural basis for a possible secretion mechanism of the T2SS.

AB - The type II secretion system (T2SS) is a macromolecular complex spanning the inner and outer membranes of Gram-negative bacteria. Remarkably, the T2SS secretes folded proteins, including multimeric assemblies such as cholera toxin and heat-labile enterotoxin from Vibrio cholerae and enterotoxigenic Escherichia coli, respectively. The major outer membrane T2SS protein is the 'secretin' GspD. Cryo-EM reconstruction of the V. cholerae secretin at 19-Å resolution reveals a dodecameric structure reminiscent of a barrel, with a large channel at its center that contains a closed periplasmic gate. The GspD periplasmic domain forms a vestibule with a conserved constriction, and it binds to a pentameric exoprotein and to the trimeric tip of the T2SS pseudopilus. By combining our results with structures of the cholera toxin and T2SS pseudopilus tip, we provide a structural basis for a possible secretion mechanism of the T2SS.

UR - https://www.scopus.com/pages/publications/77957813869

UR - https://www.scopus.com/pages/publications/77957813869#tab=citedBy

U2 - 10.1038/nsmb.1910

DO - 10.1038/nsmb.1910

M3 - Article

C2 - 20852644

AN - SCOPUS:77957813869

SN - 1545-9993

VL - 17

SP - 1226

EP - 1232

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

IS - 10

ER -

Structure of the cholera toxin secretion channel in its closed state

Abstract

Funding

UN SDGs

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this