Structure of the mouse Saa4 gene and its linkage to the serum amyloid A gene family

Maria C. De Beer, Frederick C. De Beer, Connie J. Gerardot, Darin R. Cecil, Nancy R. Webb, Michael L. Goodson, Mark S. Kindy

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The serum amyloid A (SAA) proteins are a polymorphic family of apolipoproteins associated with high-density lipoproteins (HDL). Three distinct subfamilies have been identified: (i) a cytokine-induced acute phase subfamily that is hepatically produced and can become the major apolipoprotein on HDL (SAA1, SAA2); (ii) a peripherally produced acute phase SAA3 that is only a minor HDL apolipoprotein; and (iii) a constitutive subfamily (SAA4) that is a minor normal HDL apolipoprotein comprising more than 90% of the SAA during homeostasis. Here we define the structure of the Saa4 gene. Similar to other Saa family members, it has four exons and three introns. It is 4588 bp long from the transcription start site to the end of the 3'-untranslated region and is approximately 20% larger than other Saa genes. We have located Saa4 11 kb upstream from Saa3 and 5 kb downstream from Saa1, with the pseudogene approximately 1 kb from the 5' end of Saa4. Saa4 has the same orientation as most other Saa family members, with only Saa2 having an opposing orientation. These data promote our understanding of the evolution of the Saa family. They enhance our ability to develop the mouse as a transgenic and gene deletion model to advance the understanding of the function of these apolipoproteins.

Original languageEnglish
Pages (from-to)139-142
Number of pages4
JournalGenomics
Volume34
Issue number1
DOIs
StatePublished - May 15 1996

Bibliographical note

Funding Information:
We thank Dr. Yamamoto for initially providing phage clones containing the Saa1 and Saa2 genes. This work was supported by NIH Grants AG-10886 (F. C. de Beer), AG-12981, NS31220, and AG09690 (M. S. Kindy); Council for Tobacco Research (F. C. de Beer); and Veterans Administration Research Funds (F. C. de Beer).

ASJC Scopus subject areas

  • Genetics

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