Structure, organization, and chromosomal mapping of the gene encoding macrosialin, a macrophage-restricted protein

Zhiming Jiang, Diana M. Shih, Yu Rong Xia, Aldons J. Lusis, Frederick C. De Beer, Willem J.S. De Villiers, Deneys R. Van Der Westhuyzen, Maria C. De Beer

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Murine macrosialin and its human homologue CD68 are heavily glycosylated transmembrane proteins expressed specifically in macrophages and macrophage- related cells. Macrosialin is predominantly a late endosomal protein but is also found on the cell surface where it binds oxidized LDL, an important factor in atherogenesis. We have cloned and sequenced the murine macrosialin gene (Cd68) and localized it by linkage analysis to chromosome 11. The gene is 1908 nucleotides long from the start site of transcription to the end of the 3'UTR. It has six exons, which range in size from 79 to 434 nucleotides. The promoter lacks a classical TATA box but contains other protein binding sites consistent with preferential monocyte/macrophage gene expression. Although the function of macrosialin is unknown, it might play a role in lipoprotein regulation given its binding of oxidized LDL in vitro and its colocalization to a region on chromosome 11 involved in the control of HDL levels.

Original languageEnglish
Pages (from-to)199-205
Number of pages7
JournalGenomics
Volume50
Issue number2
DOIs
StatePublished - Jun 1 1998

Bibliographical note

Funding Information:
This work was supported in part by National Institutes of Health Grant HL30568 (A.J.L.).

Keywords

  • CD68
  • Gene structure
  • Macrophage
  • Macrosialin

ASJC Scopus subject areas

  • Genetics

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