TY - JOUR
T1 - Structures of EccB1 and EccD1 from the core complex of the mycobacterial ESX-1 type VII secretion system
AU - Wagner, Jonathan M.
AU - Chan, Sum
AU - Evans, Timothy J.
AU - Kahng, Sara
AU - Kim, Jennifer
AU - Arbing, Mark A.
AU - Eisenberg, David
AU - Korotkov, Konstantin V.
N1 - Publisher Copyright:
© 2016 Wagner et al.
PY - 2016/2/27
Y1 - 2016/2/27
N2 - Background: The ESX-1 type VII secretion system is an important determinant of virulence in pathogenic mycobacteria, including Mycobacterium tuberculosis. This complicated molecular machine secretes folded proteins through the mycobacterial cell envelope to subvert the host immune response. Despite its important role in disease very little is known about the molecular architecture of the ESX-1 secretion system. Results: This study characterizes the structures of the soluble domains of two conserved core ESX-1 components - EccB1 and EccD1. The periplasmic domain of EccB1 consists of 4 repeat domains and a central domain, which together form a quasi 2-fold symmetrical structure. The repeat domains of EccB1 are structurally similar to a known peptidoglycan binding protein suggesting a role in anchoring the ESX-1 system within the periplasmic space. The cytoplasmic domain of EccD1has a ubiquitin-like fold and forms a dimer with a negatively charged groove. Conclusions: These structures represent a major step towards resolving the molecular architecture of the entire ESX-1 assembly and may contribute to ESX-1 targeted tuberculosis intervention strategies.
AB - Background: The ESX-1 type VII secretion system is an important determinant of virulence in pathogenic mycobacteria, including Mycobacterium tuberculosis. This complicated molecular machine secretes folded proteins through the mycobacterial cell envelope to subvert the host immune response. Despite its important role in disease very little is known about the molecular architecture of the ESX-1 secretion system. Results: This study characterizes the structures of the soluble domains of two conserved core ESX-1 components - EccB1 and EccD1. The periplasmic domain of EccB1 consists of 4 repeat domains and a central domain, which together form a quasi 2-fold symmetrical structure. The repeat domains of EccB1 are structurally similar to a known peptidoglycan binding protein suggesting a role in anchoring the ESX-1 system within the periplasmic space. The cytoplasmic domain of EccD1has a ubiquitin-like fold and forms a dimer with a negatively charged groove. Conclusions: These structures represent a major step towards resolving the molecular architecture of the entire ESX-1 assembly and may contribute to ESX-1 targeted tuberculosis intervention strategies.
KW - ESX
KW - EccB
KW - EccD
KW - Mycobacterium tuberculosis
KW - Type VII secretion system
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U2 - 10.1186/s12900-016-0056-6
DO - 10.1186/s12900-016-0056-6
M3 - Article
C2 - 26922638
AN - SCOPUS:84959179927
SN - 1472-6807
VL - 16
JO - BMC Structural Biology
JF - BMC Structural Biology
IS - 1
M1 - 5
ER -