Studies were undertaken to determine the cellular localization of the cyclic adenosine monophosphate (cAMP) response of various forebrain regions to β-adrenoceptor stimulation. Using brain slices, it was found that the gliotoxin, fluorocitrate (FC), which blocks metabolism selectively in glial cells, virtually abolished the cAMP response to β-receptor stimulation whereas the neurotoxin, kainic acid (KA), was without effect. FC was confirmed by electrophysiological recording to be selective for glial cells in the brain slices. Similar results were found for these agents on in vivo brain cAMP responses to β-receptor stimulation using a new microdialysis technique to measure in vivo responses. It is concluded that the cAMP response to β-adrenoceptor stimulation in various regions of the forebrain occurs predominantly in glia. To determine if this could be correlated with a second biochemical response to β-receptor stimulation, preliminary studies were undertaken on the localization of the immediate early gene, c-fos, produced in the brain after in vivo stimulation of β-receptors. It was found that unlike the cAMP responses the c-fos response to β-receptor stimulation occurs predominantly in neurons. The possible relationship of these two responses is discussed.
|Number of pages
|Brain Research Bulletin
|Published - 1992
Bibliographical noteFunding Information:
This work was supported in part by Grants MH45265, MH08618. and AFOSR 89-0208.
- β-Adrenoceptor cAMP Localization Brain slice Microdialysis Fluorocitrate Kainic acid
ASJC Scopus subject areas
- Neuroscience (all)