Studies on the substrate specificity of Escherichia coli galactokinase

Jie Yang, Xun Fu, Qiang Jia, Jie Shen, John B. Biggins, Jiqing Jiang, Jingjing Zhao, Joshua J. Schmidt, Peng G. Wang, Jon S. Thorson

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

(Martix presented) In vitro glycorandomization (IVG) technology is dependent upon the ability to rapidly synthesize sugar phosphates. Compared with chemical synthesis, enzymatic (kinase) routes to sugar phosphates would be attractive for this application. This work focuses upon the development of a high-throughput colorimetric galactokinase (GalK) assay and its application toward probing the substrate specificity and kinetic parameters of Escherichia coli GalK. The demonstrated dinitrosalicylic assay should also be generally applicable to a variety of sugar-processing enzymes.

Original languageEnglish
Pages (from-to)2223-2226
Number of pages4
JournalOrganic Letters
Volume5
Issue number13
DOIs
StatePublished - Jun 26 2003

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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