Subacute exposure to N-ethyl perfluorooctanesulfonamidoethanol results in the formation of perfluorooctanesulfonate and alters superoxide dismutase activity in female rats

Wei Xie; Qian Wu; Izabela Kania-Korwel; Job C. Tharappel; Sanjay Telu; Mitchell C. Coleman; Howard P. Glauert; Kurunthachalam Kannan; S. V.S. Mariappan; Douglas R. Spitz; Jamie Weydert; Hans Joachim Lehmler

doi:10.1007/s00204-009-0450-y

Subacute exposure to N-ethyl perfluorooctanesulfonamidoethanol results in the formation of perfluorooctanesulfonate and alters superoxide dismutase activity in female rats

Wei Xie, Qian Wu, Izabela Kania-Korwel, Job C. Tharappel, Sanjay Telu, Mitchell C. Coleman, Howard P. Glauert, Kurunthachalam Kannan, S. V.S. Mariappan, Douglas R. Spitz, Jamie Weydert, Hans Joachim Lehmler

Pharmacology and Nutritional Sciences

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Perfluorooctanesulfonamides, such as N-ethyl perfluorooctanesulfonamidoethanol (N-EtFOSE), are large scale industrial chemicals but their disposition and toxicity are poorly understood despite significant human exposure. The hypothesis that subacute exposure to N-EtFOSE, a weak peroxisome proliferator, causes a redox imbalance in vivo was tested using the known peroxisome proliferator, ciprofibrate, as a positive control. Female Sprague-Dawley rats were treated orally with N-EtFOSE, ciprofibrate or corn oil (vehicle) for 21 days, and levels of N-EtFOSE and its metabolites as well as markers of peroxisome proliferation and oxidative stress were assessed in serum, liver and/or uterus. The N-EtFOSE metabolite profile in liver and serum was in good agreement with reported in vitro biotransformation pathways in rats and the metabolite levels decreasing in the order perfluorooctanesulfonate ≫ perfluorooctanesulfonamide ̃ N-ethyl perfluorooctanesulfonamidoacetate ≫ perfluorooctanesulfonamidoethanol ̃ N-EtFOSE. Although N-EtFOSE treatment significantly decreased the growth rate, increased relative liver weight and activity of superoxide dismutases (SOD) in liver and uterus (total SOD, CuZnSOD and MnSOD), a metabolic study revealed no differences in the metabolome in serum from N-EtFOSE-treated and control animals. Ciprofibrate treatment increased liver weight and peroxisomal acyl Co-A oxidase activity in the liver and altered antioxidant enzyme activities in the uterus and liver. According to NMR metabolomic studies, ciprofibrate treated animals had altered serum lipid profiles compared to N-EtFOSE-treated and control animals, whereas putative markers of peroxisome proliferation in serum were not affected. Overall, this study demonstrates the biotransformation of N-EtFOSE to PFOS in rats that is accompanied by N-EtFOSE-induced alterations in antioxidant enzyme activity.

Original language	English
Pages (from-to)	909-924
Number of pages	16
Journal	Archives of Toxicology
Volume	83
Issue number	10
DOIs	https://doi.org/10.1007/s00204-009-0450-y
State	Published - 2009

Bibliographical note

Funding Information:
Acknowledgments We thank Brett A. Wagner, Garry R. Buettner and The University of Iowa ESR Facility for invaluable support and Rama Rao V.V.V.N.S. for the synthesis of the per-fluorooctanesulfonamides. The work was supported by a grant from the University of Iowa Center for Health Effects of Environmental Contamination (CHEEC). Additional support was provided by grants ES05605, ES013661 and ES012475 from the National Institute of Environmental Health Sciences, NIH, and by the Kentucky Agricultural Experiment Station. DRS and MCC were supported by NIH P30-CA086862.

Keywords

Metabolomics
Perfluorooctanesulfonamides
Perfluorooctanesulfonate
Peroxisomal acyl Co-A oxidase
Superoxide dismutase

ASJC Scopus subject areas

Toxicology
Health, Toxicology and Mutagenesis

Access to Document

10.1007/s00204-009-0450-y

Cite this

Xie, W., Wu, Q., Kania-Korwel, I., Tharappel, J. C., Telu, S., Coleman, M. C., Glauert, H. P., Kannan, K., Mariappan, S. V. S., Spitz, D. R., Weydert, J., & Lehmler, H. J. (2009). Subacute exposure to N-ethyl perfluorooctanesulfonamidoethanol results in the formation of perfluorooctanesulfonate and alters superoxide dismutase activity in female rats. Archives of Toxicology, 83(10), 909-924. https://doi.org/10.1007/s00204-009-0450-y

@article{b101eb592bca4086a31572dbc4a6b9a4,

title = "Subacute exposure to N-ethyl perfluorooctanesulfonamidoethanol results in the formation of perfluorooctanesulfonate and alters superoxide dismutase activity in female rats",

abstract = "Perfluorooctanesulfonamides, such as N-ethyl perfluorooctanesulfonamidoethanol (N-EtFOSE), are large scale industrial chemicals but their disposition and toxicity are poorly understood despite significant human exposure. The hypothesis that subacute exposure to N-EtFOSE, a weak peroxisome proliferator, causes a redox imbalance in vivo was tested using the known peroxisome proliferator, ciprofibrate, as a positive control. Female Sprague-Dawley rats were treated orally with N-EtFOSE, ciprofibrate or corn oil (vehicle) for 21 days, and levels of N-EtFOSE and its metabolites as well as markers of peroxisome proliferation and oxidative stress were assessed in serum, liver and/or uterus. The N-EtFOSE metabolite profile in liver and serum was in good agreement with reported in vitro biotransformation pathways in rats and the metabolite levels decreasing in the order perfluorooctanesulfonate ≫ perfluorooctanesulfonamide{\~ } N-ethyl perfluorooctanesulfonamidoacetate ≫ perfluorooctanesulfonamidoethanol{\~ } N-EtFOSE. Although N-EtFOSE treatment significantly decreased the growth rate, increased relative liver weight and activity of superoxide dismutases (SOD) in liver and uterus (total SOD, CuZnSOD and MnSOD), a metabolic study revealed no differences in the metabolome in serum from N-EtFOSE-treated and control animals. Ciprofibrate treatment increased liver weight and peroxisomal acyl Co-A oxidase activity in the liver and altered antioxidant enzyme activities in the uterus and liver. According to NMR metabolomic studies, ciprofibrate treated animals had altered serum lipid profiles compared to N-EtFOSE-treated and control animals, whereas putative markers of peroxisome proliferation in serum were not affected. Overall, this study demonstrates the biotransformation of N-EtFOSE to PFOS in rats that is accompanied by N-EtFOSE-induced alterations in antioxidant enzyme activity.",

keywords = "Metabolomics, Perfluorooctanesulfonamides, Perfluorooctanesulfonate, Peroxisomal acyl Co-A oxidase, Superoxide dismutase",

author = "Wei Xie and Qian Wu and Izabela Kania-Korwel and Tharappel, {Job C.} and Sanjay Telu and Coleman, {Mitchell C.} and Glauert, {Howard P.} and Kurunthachalam Kannan and Mariappan, {S. V.S.} and Spitz, {Douglas R.} and Jamie Weydert and Lehmler, {Hans Joachim}",

note = "Funding Information: Acknowledgments We thank Brett A. Wagner, Garry R. Buettner and The University of Iowa ESR Facility for invaluable support and Rama Rao V.V.V.N.S. for the synthesis of the per-fluorooctanesulfonamides. The work was supported by a grant from the University of Iowa Center for Health Effects of Environmental Contamination (CHEEC). Additional support was provided by grants ES05605, ES013661 and ES012475 from the National Institute of Environmental Health Sciences, NIH, and by the Kentucky Agricultural Experiment Station. DRS and MCC were supported by NIH P30-CA086862.",

year = "2009",

doi = "10.1007/s00204-009-0450-y",

language = "English",

volume = "83",

pages = "909--924",

number = "10",

}

Xie, W, Wu, Q, Kania-Korwel, I, Tharappel, JC, Telu, S, Coleman, MC, Glauert, HP, Kannan, K, Mariappan, SVS, Spitz, DR, Weydert, J & Lehmler, HJ 2009, 'Subacute exposure to N-ethyl perfluorooctanesulfonamidoethanol results in the formation of perfluorooctanesulfonate and alters superoxide dismutase activity in female rats', Archives of Toxicology, vol. 83, no. 10, pp. 909-924. https://doi.org/10.1007/s00204-009-0450-y

TY - JOUR

T1 - Subacute exposure to N-ethyl perfluorooctanesulfonamidoethanol results in the formation of perfluorooctanesulfonate and alters superoxide dismutase activity in female rats

AU - Xie, Wei

AU - Wu, Qian

AU - Kania-Korwel, Izabela

AU - Tharappel, Job C.

AU - Telu, Sanjay

AU - Coleman, Mitchell C.

AU - Glauert, Howard P.

AU - Kannan, Kurunthachalam

AU - Mariappan, S. V.S.

AU - Spitz, Douglas R.

AU - Weydert, Jamie

AU - Lehmler, Hans Joachim

N1 - Funding Information: Acknowledgments We thank Brett A. Wagner, Garry R. Buettner and The University of Iowa ESR Facility for invaluable support and Rama Rao V.V.V.N.S. for the synthesis of the per-fluorooctanesulfonamides. The work was supported by a grant from the University of Iowa Center for Health Effects of Environmental Contamination (CHEEC). Additional support was provided by grants ES05605, ES013661 and ES012475 from the National Institute of Environmental Health Sciences, NIH, and by the Kentucky Agricultural Experiment Station. DRS and MCC were supported by NIH P30-CA086862.

PY - 2009

Y1 - 2009

N2 - Perfluorooctanesulfonamides, such as N-ethyl perfluorooctanesulfonamidoethanol (N-EtFOSE), are large scale industrial chemicals but their disposition and toxicity are poorly understood despite significant human exposure. The hypothesis that subacute exposure to N-EtFOSE, a weak peroxisome proliferator, causes a redox imbalance in vivo was tested using the known peroxisome proliferator, ciprofibrate, as a positive control. Female Sprague-Dawley rats were treated orally with N-EtFOSE, ciprofibrate or corn oil (vehicle) for 21 days, and levels of N-EtFOSE and its metabolites as well as markers of peroxisome proliferation and oxidative stress were assessed in serum, liver and/or uterus. The N-EtFOSE metabolite profile in liver and serum was in good agreement with reported in vitro biotransformation pathways in rats and the metabolite levels decreasing in the order perfluorooctanesulfonate ≫ perfluorooctanesulfonamide ̃ N-ethyl perfluorooctanesulfonamidoacetate ≫ perfluorooctanesulfonamidoethanol ̃ N-EtFOSE. Although N-EtFOSE treatment significantly decreased the growth rate, increased relative liver weight and activity of superoxide dismutases (SOD) in liver and uterus (total SOD, CuZnSOD and MnSOD), a metabolic study revealed no differences in the metabolome in serum from N-EtFOSE-treated and control animals. Ciprofibrate treatment increased liver weight and peroxisomal acyl Co-A oxidase activity in the liver and altered antioxidant enzyme activities in the uterus and liver. According to NMR metabolomic studies, ciprofibrate treated animals had altered serum lipid profiles compared to N-EtFOSE-treated and control animals, whereas putative markers of peroxisome proliferation in serum were not affected. Overall, this study demonstrates the biotransformation of N-EtFOSE to PFOS in rats that is accompanied by N-EtFOSE-induced alterations in antioxidant enzyme activity.

AB - Perfluorooctanesulfonamides, such as N-ethyl perfluorooctanesulfonamidoethanol (N-EtFOSE), are large scale industrial chemicals but their disposition and toxicity are poorly understood despite significant human exposure. The hypothesis that subacute exposure to N-EtFOSE, a weak peroxisome proliferator, causes a redox imbalance in vivo was tested using the known peroxisome proliferator, ciprofibrate, as a positive control. Female Sprague-Dawley rats were treated orally with N-EtFOSE, ciprofibrate or corn oil (vehicle) for 21 days, and levels of N-EtFOSE and its metabolites as well as markers of peroxisome proliferation and oxidative stress were assessed in serum, liver and/or uterus. The N-EtFOSE metabolite profile in liver and serum was in good agreement with reported in vitro biotransformation pathways in rats and the metabolite levels decreasing in the order perfluorooctanesulfonate ≫ perfluorooctanesulfonamide ̃ N-ethyl perfluorooctanesulfonamidoacetate ≫ perfluorooctanesulfonamidoethanol ̃ N-EtFOSE. Although N-EtFOSE treatment significantly decreased the growth rate, increased relative liver weight and activity of superoxide dismutases (SOD) in liver and uterus (total SOD, CuZnSOD and MnSOD), a metabolic study revealed no differences in the metabolome in serum from N-EtFOSE-treated and control animals. Ciprofibrate treatment increased liver weight and peroxisomal acyl Co-A oxidase activity in the liver and altered antioxidant enzyme activities in the uterus and liver. According to NMR metabolomic studies, ciprofibrate treated animals had altered serum lipid profiles compared to N-EtFOSE-treated and control animals, whereas putative markers of peroxisome proliferation in serum were not affected. Overall, this study demonstrates the biotransformation of N-EtFOSE to PFOS in rats that is accompanied by N-EtFOSE-induced alterations in antioxidant enzyme activity.

KW - Metabolomics

KW - Perfluorooctanesulfonamides

KW - Perfluorooctanesulfonate

KW - Peroxisomal acyl Co-A oxidase

KW - Superoxide dismutase

UR - http://www.scopus.com/inward/record.url?scp=70349615595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349615595&partnerID=8YFLogxK

U2 - 10.1007/s00204-009-0450-y

DO - 10.1007/s00204-009-0450-y

M3 - Article

C2 - 19544052

AN - SCOPUS:70349615595

SN - 0340-5761

VL - 83

SP - 909

EP - 924

JO - Archives of Toxicology

JF - Archives of Toxicology

IS - 10

ER -

Subacute exposure to N-ethyl perfluorooctanesulfonamidoethanol results in the formation of perfluorooctanesulfonate and alters superoxide dismutase activity in female rats

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this