Subacute oxidative stress and glial reactivity in the amygdala are associated with increased anxiety following blast neurotrauma

Venkata Siva Sai Sujith Sajja, William B. Hubbard, Pamela J. VandeVord

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Behavioral symptoms, such as anxiety, are widely reported after blast overpressure (BOP) exposure. Amygdalar vulnerability to increasing magnitudes of BOP has not been investigated, and single exposures to blast have been limited to acute (G72 h) assessment. Rats were exposed to a single low, moderate, or high BOP (10, 14, or 24 psi) with an advanced blast simulator to test the susceptibility of the amygdala. Anxiety-like behavior was observed in the low- and moderate-pressure groups when subjected to the light/dark box assessment 7 days after the blast but not in high-pressure group. Immunohistochemistry was performed to measure apoptosis (cleaved caspase-3), neuronal loss (NeuN), reactive astrocytes (glial fibrillary acidic protein), microglia (Iba-1), and oxidative stress (CuZn superoxide dismutase). Slower progression of injury cascades was associated with a significant increase in anxiety, apoptosis, and astrogliosis in the low pressure group compared with others. A significant increase of CuZn superoxide dismutase in the low pressure group could be associated with neuroprotection from cell death caused by oxidative stress because neuronal loss was significant in the moderate- and high- but not the low-pressure group. Overall, this study demonstrated that overpressure as low as 10 psi can induce subacute anxiety, in addition to neuropathologic changes in the amygdala.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
StatePublished - Aug 2015

Bibliographical note

Publisher Copyright:
Copyright © 2014 by the Shock Society.


  • Amygdala
  • Anxiety
  • Apoptosis
  • Blast
  • Inflammation
  • Oxidative stress
  • Trauma

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine


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